ANA 812

Anatomy & Neurobiology

ANA 812Lab Session 11

Peripheral Blood

Bryan GuilloryLashaundra ThedfordPhilip OverallJohn Watson


Slide #42: Human Blood (Wright-Giemsa)

Normal Blood (100X)



Platelets (Thrombocytes) (1000x)

Platelets

● Small (2-4 μm)● Granular



Erythrocytes (RBC’s)Normal Blood (400X)

Erythrocytes

-Biconcave Discs

-Central Pallor



Normal Blood Erythrocytes (1000X)

•Biconcave Discs

•Central Pallor

•7-8 um in diameter


Slide #43: Abnormal Human Blood (Wright-Giemsa)

Anemic Blood (100X)

•Smaller RBC’s

•Larger Central Pallors

•Variable Size and Shape



Anemic Blood (400X)

•Smaller RBC’s

•Larger Central Pallors




Anemic Blood (1000X)

• Various Sized RBC’s

•Larger Central Pallor



Slide #42: Human Blood (Wright-Giemsa)Granulocytes: Neutrophils (400X)

•Neutrophils

•Granular Appearance


Slide #42: Human Blood (Wright-Giemsa)Banded Neutrophils (400X)

•“Stab Forms”

•Nucleus Elongated

•Less Prominent than Segmented Forms



Anemic Blood (1000X)Segmented Neutrophil

•PMNs

•Lobed Nucleus w/a Fine Filament Between

•Most common Neutrophil



Agranulocytes: Lymphocytes (1000x)

Lymphocyte

● Round Nucleus● Cytoplasm (blue halo)



Agranulocytes: Monocytes (1000x)

Monocyte

● Horseshoe, various shaped nucleus

● Large (14-20 μm)



Granulocytes: Eosinophil (1000x)

Eosinophil

Bi-lobed nucleus

Granules


Electron MicrographGranulocytes: Eosinophil

Lysosomal crystals

Granule

Nucleus



Granulocytes: Basophil (1000x)

Basophil

● “baso” because of blue-black granules when stained

● large granules that overlie nucleus (histamine)

● nucleus has smudged, indistinct chromatin pattern


Slide #41: Peripheral Blood Film – Human (Wright-Giemsa)

400x

•Monocyte

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