Bacteria presentation
Transcript of Bacteria presentation
ISOLATING BACTERIA FROM TROPICAL CONTAMINATED
SOILS IN PUERTO RICO RISE SPRING 2015
Sara DiazPatricia Rodriguez
Camille RuizJustin Cotto
SAMPLING LOCATIONS
Sample Coordinates
Environmental description
Location
Date/Time
Ruiz C- #1
18°6’55.1”N; 66°9’36.2”W
Moist (underneath grass),
5 cm; 72°F
Cayey, PR
January/28/15
7:30 p.m.
Cotto J- #2
18.15º N66.18º W
Moist environment (above grass), 76ºF
Cidra, PR
January/29/15
8:00 a.m.
SAMPLING LOCATIONSSample Coordinates Environmental
descriptionLocation Date/
Time
Diaz S-#1
18°15'15.7"N 66°06'06.9"W
Moist environment80°F
Aguas Buenas,
P.R.
January/27/15
4:26 p.m.
Rodríguez P-#2
18°15'15.7"N 66°06'06.9"W
Moist environment 80°F
Aguas Buenas,
P.R.
January/27/15
4:26 p.m.
RESULTS FROM DIFFERENT PROCEDURES
Process CLRV30TSA01A CLRV30M01B JCR30TSA01A
Gram Stain Negative Positive Positive
Bacterial Morphology Coccus Bacillus Bacillus
PCR/Electrophoresis #1
Negative Negative Negative
PCR/Electrophoresis #2
Positive Negative -
Antibiotic Production Negative Negative Negative
GRAM STAIN RESULTS
CLRV30TSA01A CLRV30M01B JCR30TSA01A
POSITIVE ELECTROPHORESIS
RESULTS FROM DIFFERENT PROCEDURESProcess S15UPRC-RISESCDM30T01B S15UPRC-RISEPR30M01
Gram Stain Positive Positive
Bacterial Morphology Bacillus Bacillus
PCR/Electrophoresis #1
Negative Negative
PCR/Electrophoresis #2
Negative Negative
Antibiotic Production Negative Negative
GRAM STAIN RESULTS
S15UPRC-RISEPR30M01 S15UPRC-RISESCDM30T01B
ANTIBIOTIC RESISTANCE TEST RESULTS
Antibiotic CLRV30TSA01A CLRV30M01B JCR30TSA01A
Kanamycin 2.0 cm 1.0 cm 2.0 cm
Penicillin 0.0 cm 0.0 cm -
Tetracycline 1.6 cm 2.4 cm 2.2 cm
Gentamicin 1.9 cm 0.9 cm -
Chloramphenicol 0.0 cm 0.0 cm 2.5 cm
Bacitracin 0.0 cm 0.0 cm 1.5 cm
Vancomycin 0.0 cm 0.0 cm -
Streptomycin 1.6 cm 1.0 cm 1.9 cm
ANTIBIOTIC RESISTANCE TEST RESULTS
Antibiotic S15UPRC-RISESCDM30T01B
Streptomycin 2.2 cm
Chloramphenicol 2.3 cm
Erythromycin 1.0 cm
Kanamycin 1.6 cm
Gentamicin 0.6 cm
Antibiotic S15UPRC-RISEPR30M01
Rifampin 0.13 cm
Kanamycin 0.16 cm
Vancomycin 0.17cm
Streptomycin 0.30 cm
Tetracyclin 0.14 cm