ISOLATING BACTERIA FROM TROPICAL CONTAMINATED SOILS IN PUERTO RICO

ISOLATING BACTERIA FROM TROPICAL CONTAMINATED SOILS IN PUERTO RICO RISE SPRING 2015Sara DiazPatricia RodriguezCamille RuizJustin Cotto

SAMPLING LOCATIONS

SampleCoordinatesEnvironmental descriptionLocationDate/TimeRuiz C- #118655.1N; 66936.2WMoist (underneath grass), 5 cm; 72FCayey, PRJanuary/28/157:30 p.m.Cotto J- #218.15 N66.18 WMoist environment (above grass), 76FCidra, PRJanuary/29/158:00 a.m.

SAMPLING LOCATIONS

SampleCoordinatesEnvironmental descriptionLocationDate/TimeDiaz S-#11815'15.7"N 6606'06.9"WMoist environment80FAguas Buenas, P.R.January/27/154:26 p.m.Rodrguez P-#21815'15.7"N 6606'06.9"WMoist environment 80FAguas Buenas, P.R.January/27/154:26 p.m.

Results from different proceduresProcessCLRV30TSA01ACLRV30M01BJCR30TSA01AGram StainNegativePositivePositiveBacterial MorphologyCoccusBacillusBacillusPCR/Electrophoresis #1NegativeNegativeNegativePCR/Electrophoresis #2PositiveNegative-Antibiotic ProductionNegativeNegativeNegative

Gram stain results

CLRV30TSA01A CLRV30M01B JCR30TSA01A

Positive electrophoresis

Results from different proceduresProcessS15UPRC-RISESCDM30T01BS15UPRC-RISEPR30M01Gram StainPositivePositiveBacterial MorphologyBacillusBacillusPCR/Electrophoresis #1NegativeNegativePCR/Electrophoresis #2NegativeNegativeAntibiotic ProductionNegativeNegative

Gram Stain resultsS15UPRC-RISEPR30M01 S15UPRC-RISESCDM30T01B

ANTIBIOTIC RESISTANCE TEST RESULTSAntibioticCLRV30TSA01ACLRV30M01BJCR30TSA01AKanamycin2.0 cm1.0 cm2.0 cmPenicillin0.0 cm0.0 cm-Tetracycline1.6 cm2.4 cm 2.2 cmGentamicin1.9 cm0.9 cm-Chloramphenicol0.0 cm0.0 cm2.5 cmBacitracin0.0 cm0.0 cm1.5 cmVancomycin0.0 cm0.0 cm-Streptomycin1.6 cm1.0 cm1.9 cm

ANTIBIOTIC RESISTANCE TEST RESULTSAntibioticS15UPRC-RISESCDM30T01B

Streptomycin2.2 cmChloramphenicol2.3 cmErythromycin1.0 cmKanamycin1.6 cmGentamicin0.6 cm

AntibioticS15UPRC-RISEPR30M01Rifampin0.13 cmKanamycin0.16 cmVancomycin0.17cmStreptomycin0.30 cmTetracyclin0.14 cm