Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP...

49
Morfometría, Morphometrics Postgrado en Geología Asignatura de Master Metodología y técnicas de campo y laboratorio en investigación paleontológica 60331 José Ignacio Canudo y Gloria Cuenca-Bescós

Transcript of Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP...

Page 1: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Morfometriacutea Morphometrics

bull Postgrado en Geologiacutea

bull Asignatura de Master Metodologiacutea y teacutecnicas de campo y laboratorio en investigacioacuten paleontoloacutegica 60331

bull Joseacute Ignacio Canudo y Gloria Cuenca-Bescoacutes

Introduccioacuten a la Morfometriacutea

geomeacutetrica en Paleontologia

bull Profesores Joseacute Ignacio Canudo y Gloria

Cuenca-Bescoacutes

bull Web wwwaragosauruscomdocenciamaster

iquestQue es la morfometriacutea

bull La morfometriacutea es el estudio de la

variacioacuten de forma en covariacioacuten con

otras variables (Bookstein 1991 Dryden

and Mardia 1998 Adams et 04) como la

filogenia paleogeografiacutea biomecaacutenica

bull Es el estudio del cambio de forma con el

crecimiento o alometriacutea (Jolicoeur 1963

Adams et 04v) o con la evolucioacuten en una

liacutenea filogeneacutetica

iquestQue es morfometriacutea

bull Gingerich Ph 1990 Prediction of body size in mammalian species from long bone lengths and diameters Contributions from the Museum of Paleontolgoy University of Michigan 28

Utilizoacute la foacutermula Y = axb (donde x es la variable independiente la masa corporal e y es la variable dependiente la longitud o el diaacutemetro de un hueso largo)

bull Dean C Adams F James Rohlf and Dennis E Slice 2004 Geometric Morphometrics Ten Years of Progress Following the lsquoRevolutionrsquo Italian Journal of Zoology along with papers by the participants of the workshop held in Rome in January 2002 J Zool 71 5-16

Morfometriacutea La medida de la forma

iquestqueacute es la forma en biologiacutea bull Expresioacuten de los genes fenotipo

bull Expresioacuten de la adaptacioacuten y la historia filogeneacutetica adaptacioacuten ecoloacutegico-evolutiva

Lobo placentado

-Tarsos maacutes largos

-Funciones

trituradoras y cortantes

en distintas piezas

dentales

-Angulo de apertura de

boca menor

Lobo marsupial o

Tilacino

-Tarsos cortos

-Funcioacuten trituradora y

cortante en los

mismos molares

-Angulo de apertura

de boca mayor

-Mandiacutebula Proceso

angular acusado y

curvado hacia el lado

interno

Los dientes de los

mamiacuteferos los

arvicoacutelidos

bull Cuenca Bescoacutes et al 1995

Estiramos lat

La forma en postcraneal el feacutemur de los

sauroacutepodos

bull Canudo y Cuenca Bescoacutes 2004

iquestCoacutemo y por queacute estudiamos la

forma

Orientacioacuten y funciones de los

dientes

bull Arrancar

bull Retener

bull Peinar y

cuidar el

pelo

bull Descortezar

bull roer

bull Cortar

bull Moler

Toma de medidas

W

L

iquestCoacutemo estudiamos la forma

httplifebiosunysbedumorphindexhtml

Adquisicioacuten de los datos

bull Calibre

bull Datos tomados con otras herramientas y volcados a una hoja de caacutelculo (Excel Pasthellip)

bull Imaacutegenes yo datos a partir de la literatura

bull Imaacutegenes tomadas con caacutemara digital

bull Imaacutegenes tomadas desde un microscopio o lupa binocular

Espeacutecimen Longitud (mm)

1a 33

1b 45

1c 29

Landmarks digitalizados con tpsDig2

httplifebiosunysbedumorphindexhtml

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 2: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Introduccioacuten a la Morfometriacutea

geomeacutetrica en Paleontologia

bull Profesores Joseacute Ignacio Canudo y Gloria

Cuenca-Bescoacutes

bull Web wwwaragosauruscomdocenciamaster

iquestQue es la morfometriacutea

bull La morfometriacutea es el estudio de la

variacioacuten de forma en covariacioacuten con

otras variables (Bookstein 1991 Dryden

and Mardia 1998 Adams et 04) como la

filogenia paleogeografiacutea biomecaacutenica

bull Es el estudio del cambio de forma con el

crecimiento o alometriacutea (Jolicoeur 1963

Adams et 04v) o con la evolucioacuten en una

liacutenea filogeneacutetica

iquestQue es morfometriacutea

bull Gingerich Ph 1990 Prediction of body size in mammalian species from long bone lengths and diameters Contributions from the Museum of Paleontolgoy University of Michigan 28

Utilizoacute la foacutermula Y = axb (donde x es la variable independiente la masa corporal e y es la variable dependiente la longitud o el diaacutemetro de un hueso largo)

bull Dean C Adams F James Rohlf and Dennis E Slice 2004 Geometric Morphometrics Ten Years of Progress Following the lsquoRevolutionrsquo Italian Journal of Zoology along with papers by the participants of the workshop held in Rome in January 2002 J Zool 71 5-16

Morfometriacutea La medida de la forma

iquestqueacute es la forma en biologiacutea bull Expresioacuten de los genes fenotipo

bull Expresioacuten de la adaptacioacuten y la historia filogeneacutetica adaptacioacuten ecoloacutegico-evolutiva

Lobo placentado

-Tarsos maacutes largos

-Funciones

trituradoras y cortantes

en distintas piezas

dentales

-Angulo de apertura de

boca menor

Lobo marsupial o

Tilacino

-Tarsos cortos

-Funcioacuten trituradora y

cortante en los

mismos molares

-Angulo de apertura

de boca mayor

-Mandiacutebula Proceso

angular acusado y

curvado hacia el lado

interno

Los dientes de los

mamiacuteferos los

arvicoacutelidos

bull Cuenca Bescoacutes et al 1995

Estiramos lat

La forma en postcraneal el feacutemur de los

sauroacutepodos

bull Canudo y Cuenca Bescoacutes 2004

iquestCoacutemo y por queacute estudiamos la

forma

Orientacioacuten y funciones de los

dientes

bull Arrancar

bull Retener

bull Peinar y

cuidar el

pelo

bull Descortezar

bull roer

bull Cortar

bull Moler

Toma de medidas

W

L

iquestCoacutemo estudiamos la forma

httplifebiosunysbedumorphindexhtml

Adquisicioacuten de los datos

bull Calibre

bull Datos tomados con otras herramientas y volcados a una hoja de caacutelculo (Excel Pasthellip)

bull Imaacutegenes yo datos a partir de la literatura

bull Imaacutegenes tomadas con caacutemara digital

bull Imaacutegenes tomadas desde un microscopio o lupa binocular

Espeacutecimen Longitud (mm)

1a 33

1b 45

1c 29

Landmarks digitalizados con tpsDig2

httplifebiosunysbedumorphindexhtml

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 3: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

iquestQue es la morfometriacutea

bull La morfometriacutea es el estudio de la

variacioacuten de forma en covariacioacuten con

otras variables (Bookstein 1991 Dryden

and Mardia 1998 Adams et 04) como la

filogenia paleogeografiacutea biomecaacutenica

bull Es el estudio del cambio de forma con el

crecimiento o alometriacutea (Jolicoeur 1963

Adams et 04v) o con la evolucioacuten en una

liacutenea filogeneacutetica

iquestQue es morfometriacutea

bull Gingerich Ph 1990 Prediction of body size in mammalian species from long bone lengths and diameters Contributions from the Museum of Paleontolgoy University of Michigan 28

Utilizoacute la foacutermula Y = axb (donde x es la variable independiente la masa corporal e y es la variable dependiente la longitud o el diaacutemetro de un hueso largo)

bull Dean C Adams F James Rohlf and Dennis E Slice 2004 Geometric Morphometrics Ten Years of Progress Following the lsquoRevolutionrsquo Italian Journal of Zoology along with papers by the participants of the workshop held in Rome in January 2002 J Zool 71 5-16

Morfometriacutea La medida de la forma

iquestqueacute es la forma en biologiacutea bull Expresioacuten de los genes fenotipo

bull Expresioacuten de la adaptacioacuten y la historia filogeneacutetica adaptacioacuten ecoloacutegico-evolutiva

Lobo placentado

-Tarsos maacutes largos

-Funciones

trituradoras y cortantes

en distintas piezas

dentales

-Angulo de apertura de

boca menor

Lobo marsupial o

Tilacino

-Tarsos cortos

-Funcioacuten trituradora y

cortante en los

mismos molares

-Angulo de apertura

de boca mayor

-Mandiacutebula Proceso

angular acusado y

curvado hacia el lado

interno

Los dientes de los

mamiacuteferos los

arvicoacutelidos

bull Cuenca Bescoacutes et al 1995

Estiramos lat

La forma en postcraneal el feacutemur de los

sauroacutepodos

bull Canudo y Cuenca Bescoacutes 2004

iquestCoacutemo y por queacute estudiamos la

forma

Orientacioacuten y funciones de los

dientes

bull Arrancar

bull Retener

bull Peinar y

cuidar el

pelo

bull Descortezar

bull roer

bull Cortar

bull Moler

Toma de medidas

W

L

iquestCoacutemo estudiamos la forma

httplifebiosunysbedumorphindexhtml

Adquisicioacuten de los datos

bull Calibre

bull Datos tomados con otras herramientas y volcados a una hoja de caacutelculo (Excel Pasthellip)

bull Imaacutegenes yo datos a partir de la literatura

bull Imaacutegenes tomadas con caacutemara digital

bull Imaacutegenes tomadas desde un microscopio o lupa binocular

Espeacutecimen Longitud (mm)

1a 33

1b 45

1c 29

Landmarks digitalizados con tpsDig2

httplifebiosunysbedumorphindexhtml

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 4: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

iquestQue es morfometriacutea

bull Gingerich Ph 1990 Prediction of body size in mammalian species from long bone lengths and diameters Contributions from the Museum of Paleontolgoy University of Michigan 28

Utilizoacute la foacutermula Y = axb (donde x es la variable independiente la masa corporal e y es la variable dependiente la longitud o el diaacutemetro de un hueso largo)

bull Dean C Adams F James Rohlf and Dennis E Slice 2004 Geometric Morphometrics Ten Years of Progress Following the lsquoRevolutionrsquo Italian Journal of Zoology along with papers by the participants of the workshop held in Rome in January 2002 J Zool 71 5-16

Morfometriacutea La medida de la forma

iquestqueacute es la forma en biologiacutea bull Expresioacuten de los genes fenotipo

bull Expresioacuten de la adaptacioacuten y la historia filogeneacutetica adaptacioacuten ecoloacutegico-evolutiva

Lobo placentado

-Tarsos maacutes largos

-Funciones

trituradoras y cortantes

en distintas piezas

dentales

-Angulo de apertura de

boca menor

Lobo marsupial o

Tilacino

-Tarsos cortos

-Funcioacuten trituradora y

cortante en los

mismos molares

-Angulo de apertura

de boca mayor

-Mandiacutebula Proceso

angular acusado y

curvado hacia el lado

interno

Los dientes de los

mamiacuteferos los

arvicoacutelidos

bull Cuenca Bescoacutes et al 1995

Estiramos lat

La forma en postcraneal el feacutemur de los

sauroacutepodos

bull Canudo y Cuenca Bescoacutes 2004

iquestCoacutemo y por queacute estudiamos la

forma

Orientacioacuten y funciones de los

dientes

bull Arrancar

bull Retener

bull Peinar y

cuidar el

pelo

bull Descortezar

bull roer

bull Cortar

bull Moler

Toma de medidas

W

L

iquestCoacutemo estudiamos la forma

httplifebiosunysbedumorphindexhtml

Adquisicioacuten de los datos

bull Calibre

bull Datos tomados con otras herramientas y volcados a una hoja de caacutelculo (Excel Pasthellip)

bull Imaacutegenes yo datos a partir de la literatura

bull Imaacutegenes tomadas con caacutemara digital

bull Imaacutegenes tomadas desde un microscopio o lupa binocular

Espeacutecimen Longitud (mm)

1a 33

1b 45

1c 29

Landmarks digitalizados con tpsDig2

httplifebiosunysbedumorphindexhtml

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 5: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Morfometriacutea La medida de la forma

iquestqueacute es la forma en biologiacutea bull Expresioacuten de los genes fenotipo

bull Expresioacuten de la adaptacioacuten y la historia filogeneacutetica adaptacioacuten ecoloacutegico-evolutiva

Lobo placentado

-Tarsos maacutes largos

-Funciones

trituradoras y cortantes

en distintas piezas

dentales

-Angulo de apertura de

boca menor

Lobo marsupial o

Tilacino

-Tarsos cortos

-Funcioacuten trituradora y

cortante en los

mismos molares

-Angulo de apertura

de boca mayor

-Mandiacutebula Proceso

angular acusado y

curvado hacia el lado

interno

Los dientes de los

mamiacuteferos los

arvicoacutelidos

bull Cuenca Bescoacutes et al 1995

Estiramos lat

La forma en postcraneal el feacutemur de los

sauroacutepodos

bull Canudo y Cuenca Bescoacutes 2004

iquestCoacutemo y por queacute estudiamos la

forma

Orientacioacuten y funciones de los

dientes

bull Arrancar

bull Retener

bull Peinar y

cuidar el

pelo

bull Descortezar

bull roer

bull Cortar

bull Moler

Toma de medidas

W

L

iquestCoacutemo estudiamos la forma

httplifebiosunysbedumorphindexhtml

Adquisicioacuten de los datos

bull Calibre

bull Datos tomados con otras herramientas y volcados a una hoja de caacutelculo (Excel Pasthellip)

bull Imaacutegenes yo datos a partir de la literatura

bull Imaacutegenes tomadas con caacutemara digital

bull Imaacutegenes tomadas desde un microscopio o lupa binocular

Espeacutecimen Longitud (mm)

1a 33

1b 45

1c 29

Landmarks digitalizados con tpsDig2

httplifebiosunysbedumorphindexhtml

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 6: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

iquestqueacute es la forma en biologiacutea bull Expresioacuten de los genes fenotipo

bull Expresioacuten de la adaptacioacuten y la historia filogeneacutetica adaptacioacuten ecoloacutegico-evolutiva

Lobo placentado

-Tarsos maacutes largos

-Funciones

trituradoras y cortantes

en distintas piezas

dentales

-Angulo de apertura de

boca menor

Lobo marsupial o

Tilacino

-Tarsos cortos

-Funcioacuten trituradora y

cortante en los

mismos molares

-Angulo de apertura

de boca mayor

-Mandiacutebula Proceso

angular acusado y

curvado hacia el lado

interno

Los dientes de los

mamiacuteferos los

arvicoacutelidos

bull Cuenca Bescoacutes et al 1995

Estiramos lat

La forma en postcraneal el feacutemur de los

sauroacutepodos

bull Canudo y Cuenca Bescoacutes 2004

iquestCoacutemo y por queacute estudiamos la

forma

Orientacioacuten y funciones de los

dientes

bull Arrancar

bull Retener

bull Peinar y

cuidar el

pelo

bull Descortezar

bull roer

bull Cortar

bull Moler

Toma de medidas

W

L

iquestCoacutemo estudiamos la forma

httplifebiosunysbedumorphindexhtml

Adquisicioacuten de los datos

bull Calibre

bull Datos tomados con otras herramientas y volcados a una hoja de caacutelculo (Excel Pasthellip)

bull Imaacutegenes yo datos a partir de la literatura

bull Imaacutegenes tomadas con caacutemara digital

bull Imaacutegenes tomadas desde un microscopio o lupa binocular

Espeacutecimen Longitud (mm)

1a 33

1b 45

1c 29

Landmarks digitalizados con tpsDig2

httplifebiosunysbedumorphindexhtml

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 7: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Los dientes de los

mamiacuteferos los

arvicoacutelidos

bull Cuenca Bescoacutes et al 1995

Estiramos lat

La forma en postcraneal el feacutemur de los

sauroacutepodos

bull Canudo y Cuenca Bescoacutes 2004

iquestCoacutemo y por queacute estudiamos la

forma

Orientacioacuten y funciones de los

dientes

bull Arrancar

bull Retener

bull Peinar y

cuidar el

pelo

bull Descortezar

bull roer

bull Cortar

bull Moler

Toma de medidas

W

L

iquestCoacutemo estudiamos la forma

httplifebiosunysbedumorphindexhtml

Adquisicioacuten de los datos

bull Calibre

bull Datos tomados con otras herramientas y volcados a una hoja de caacutelculo (Excel Pasthellip)

bull Imaacutegenes yo datos a partir de la literatura

bull Imaacutegenes tomadas con caacutemara digital

bull Imaacutegenes tomadas desde un microscopio o lupa binocular

Espeacutecimen Longitud (mm)

1a 33

1b 45

1c 29

Landmarks digitalizados con tpsDig2

httplifebiosunysbedumorphindexhtml

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 8: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

La forma en postcraneal el feacutemur de los

sauroacutepodos

bull Canudo y Cuenca Bescoacutes 2004

iquestCoacutemo y por queacute estudiamos la

forma

Orientacioacuten y funciones de los

dientes

bull Arrancar

bull Retener

bull Peinar y

cuidar el

pelo

bull Descortezar

bull roer

bull Cortar

bull Moler

Toma de medidas

W

L

iquestCoacutemo estudiamos la forma

httplifebiosunysbedumorphindexhtml

Adquisicioacuten de los datos

bull Calibre

bull Datos tomados con otras herramientas y volcados a una hoja de caacutelculo (Excel Pasthellip)

bull Imaacutegenes yo datos a partir de la literatura

bull Imaacutegenes tomadas con caacutemara digital

bull Imaacutegenes tomadas desde un microscopio o lupa binocular

Espeacutecimen Longitud (mm)

1a 33

1b 45

1c 29

Landmarks digitalizados con tpsDig2

httplifebiosunysbedumorphindexhtml

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 9: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

iquestCoacutemo y por queacute estudiamos la

forma

Orientacioacuten y funciones de los

dientes

bull Arrancar

bull Retener

bull Peinar y

cuidar el

pelo

bull Descortezar

bull roer

bull Cortar

bull Moler

Toma de medidas

W

L

iquestCoacutemo estudiamos la forma

httplifebiosunysbedumorphindexhtml

Adquisicioacuten de los datos

bull Calibre

bull Datos tomados con otras herramientas y volcados a una hoja de caacutelculo (Excel Pasthellip)

bull Imaacutegenes yo datos a partir de la literatura

bull Imaacutegenes tomadas con caacutemara digital

bull Imaacutegenes tomadas desde un microscopio o lupa binocular

Espeacutecimen Longitud (mm)

1a 33

1b 45

1c 29

Landmarks digitalizados con tpsDig2

httplifebiosunysbedumorphindexhtml

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 10: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Orientacioacuten y funciones de los

dientes

bull Arrancar

bull Retener

bull Peinar y

cuidar el

pelo

bull Descortezar

bull roer

bull Cortar

bull Moler

Toma de medidas

W

L

iquestCoacutemo estudiamos la forma

httplifebiosunysbedumorphindexhtml

Adquisicioacuten de los datos

bull Calibre

bull Datos tomados con otras herramientas y volcados a una hoja de caacutelculo (Excel Pasthellip)

bull Imaacutegenes yo datos a partir de la literatura

bull Imaacutegenes tomadas con caacutemara digital

bull Imaacutegenes tomadas desde un microscopio o lupa binocular

Espeacutecimen Longitud (mm)

1a 33

1b 45

1c 29

Landmarks digitalizados con tpsDig2

httplifebiosunysbedumorphindexhtml

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 11: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Toma de medidas

W

L

iquestCoacutemo estudiamos la forma

httplifebiosunysbedumorphindexhtml

Adquisicioacuten de los datos

bull Calibre

bull Datos tomados con otras herramientas y volcados a una hoja de caacutelculo (Excel Pasthellip)

bull Imaacutegenes yo datos a partir de la literatura

bull Imaacutegenes tomadas con caacutemara digital

bull Imaacutegenes tomadas desde un microscopio o lupa binocular

Espeacutecimen Longitud (mm)

1a 33

1b 45

1c 29

Landmarks digitalizados con tpsDig2

httplifebiosunysbedumorphindexhtml

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 12: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

iquestCoacutemo estudiamos la forma

httplifebiosunysbedumorphindexhtml

Adquisicioacuten de los datos

bull Calibre

bull Datos tomados con otras herramientas y volcados a una hoja de caacutelculo (Excel Pasthellip)

bull Imaacutegenes yo datos a partir de la literatura

bull Imaacutegenes tomadas con caacutemara digital

bull Imaacutegenes tomadas desde un microscopio o lupa binocular

Espeacutecimen Longitud (mm)

1a 33

1b 45

1c 29

Landmarks digitalizados con tpsDig2

httplifebiosunysbedumorphindexhtml

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 13: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Adquisicioacuten de los datos

bull Calibre

bull Datos tomados con otras herramientas y volcados a una hoja de caacutelculo (Excel Pasthellip)

bull Imaacutegenes yo datos a partir de la literatura

bull Imaacutegenes tomadas con caacutemara digital

bull Imaacutegenes tomadas desde un microscopio o lupa binocular

Espeacutecimen Longitud (mm)

1a 33

1b 45

1c 29

Landmarks digitalizados con tpsDig2

httplifebiosunysbedumorphindexhtml

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 14: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Tps digit program

Free at Suny

httplifebiosunysbedumorph

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 15: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Dientes complejos Arvicolinae

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 16: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Iberon 25 Landmarks

1

2

4

3

5

6

7

8 9

1011

12

14

15

16

17

18

19

20

21

22

23

24

13

1

2

3

4

5

6

7

89

10

11

12

131415

16

17

18

19

20

21

22

23

24

25

25

archivo 25 iberomys landmarks

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 17: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Some extreme morphologies

Allophaiomys pliocaenicus Pirro

Nord

Early Pleistocene Italy Iberomys cabrerae

Extant Spain

Allophaiomys lavocati

Sima del Elefante

Atapuerca

Early Pleistocene (older

than the Italian species

Spain

AllophLavF

N3_1r

Early

Pleistocen

e of

Granada

similar to

Atapuerca

LM =17-14 of Iberon LM =15-14 of Iberon LM =15-14 of Iberon

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 18: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Landmarks de 90 especiacutemenes de

Microtus agrestis

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 19: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Landmarks de 90 especiacutemenes de

Microtus agrestis

Data 90 Specimens 22 Points 2 Dimensions

All specimen superimposed by GLS method

Sum of Squared Deviations = 1101217

The groups limit is after the specimen 0

PCS and scores computed

Percentages of variance for the 8 first PCS

61395977 61395977

8155754 69551731

4681460 74233190

3352225 77585415

2619139 80204554

2500418 82704973

2235576 84940549

1789387 86729936

Multivariate regression coefficients (normalized) with 3 PCS

PCS 1 0998142

PCS 2 -0057223

PCS 3 -0020926

Regression statistic for 3 components

Rsup2= 0227471 F= 8244611 with 3 and 86 DF plt 0000070

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 20: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Plot de 90 especiacutemenes de

Microtus agrestis

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 21: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Distribucioacuten estratigraacutefica de los roedores del cuaternario de Gran Dolina Atapuerca Burgos

0 m

Allo

ph

aio

mys

Mim

om

ys

Ibe

rom

ys

Terr

ico

la

Ste

no

cra

niu

s

Arv

ico

la

Mic

rom

ys

Hy

str

ix

Ca

sto

r

Ma

rmo

ta

Plio

mys

Mic

rotu

s

Allo

cri

cetu

s

Ap

od

em

us

Elio

mys

TD7

TD8

TD10

TD11

TD6

TD5

TD34

TD2

TD1

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 22: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Procustes analisis APS

bull Procuste fue un ladroacuten que bien descuartizaba o bien estiraba a sus viacutectimas para ajustarlas al tamantildeo de su cama de ahiacute viene la frase hecha de ldquola cama Procusterdquo que se aplica sobre todo en poliacutetica la censura o para prestaciones puacuteblicas en las que es el usuario el que debe adaptarse y no al reveacuteshellip

bull The APS package

bull The shape analysis softwares APS ApsDigit and ApsUtil are written in English and always free for the students Aps combines Procuste superposition and statistical calculations ApsDigit is used locate landmarks on digital images (JPEG Bitmap tiff GIF) ApsUtil is used to handle files (Selection of points individuals change of classification)

bull httpwwwprocustecom

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 23: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

APS

APS est un logiciel danalyse quantitative de la

variabiliteacute morphologique des formes vivantes La

meacutethode utiliseacutee est la superposition procruste

geacuteneacuteraliseacutee

Destineacute aux biologistes et aux cliniciens APS prend en

charge les aspects matheacutematiques et statistiques de

lanalyse de forme Lanalyse se fait en 2 temps

1 Une superposition procruste de lensemble des

speacutecimens

2 Une analyse statistique qui visualise et teste la covariance

entre dune part la conformation et dautre part un facteur

biologique comme la taille lappartenance agrave groupe la

preacutesence ou non dune pathologie

Il est conccedilu pour eacutetudier un ou deux groupes

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 24: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

APS Digitalizar landmarks

DigitX is designed to digitize landmarks on Jpeg or Bitmap picture

middot Select the total number of landmarks to digitize with the second

Combo-Box of the toolbar

middot Select a picture in the graphic file selector on the left

middot Click on to load the link file (optional)

middot Click on to load the consensus file (optional)

middot Digitize landmarks using the left button of the mouse

middot To cancel last landmark position by a click on undo arrow

middot When all landmarks are digitized save the file by a click on save file

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 25: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

APS Crear archivo

Getting started with ApsUtil

ApsUtil create a data file (dat) with specimen ordered by

addition of smaller data files Input and output files are to

APS data format with an header line (number of specimens

number

of landmarks number of dimensions) followed by landmarks

coordinates

NEW button clear all previous data and begin a new data file

ADD button add a file containing one or many specimen

MIX button add a new file to a previous one by mixing the

two file

This ordering of specimen is required before to compute

shape differences

When file is saved specimens are ordered like

specimen 1 of the 1st file

specimen 1 of the 2nd file

specimen 2 of the 1st file

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 26: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

APS analisis

bull New features of Version 241

bull A single specimen or all a sample can be superimposed on any reloaded

bull specimen as consensus (File -gt Reload -gt Consensus)

bull Shape vectors (eg discriminant vectors computed with other software)

bull can be reloaded for visualization (File -gt Reload -gt Pcs)

bull The shape vector must be of norm 1

bull Projections of specimens on shape vectors are automatically computed

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 27: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Otros

elementos del

esqueleto

A Antarctosaurus wichmannianus B

Saltasaurus loricatus C Neuquensaurus

australis D Neuquensaurus robustus E

Lirainosaurus astibiae F Ampelosaurus atacis

G Magyarosaurus dacus H Magyarosaurus

dacus Jianu I Alamosaurus sanjuanensis J

Aegyptosaurus baharijiensis K

Opisthocoelicaudia skarzynskii

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 28: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Canudo amp Cuenca Bescos 2004

wwwaragosauruscom

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 29: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Aegyptosaurus Alamosaurus

Imaacutegenes literatura

Fotos ejemplares

Hacer un poliacutegono con los landmarks

Guardar la misma posicioacuten (en una liacutenea basal por ej)

APS digit

APS util

APS program CP

Opisthocoelicaudia

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 30: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Resultados

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 31: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

APS Fecha Thu 21 Sep 2006 213918

+0200 [210906 213918 CEST]

De Info Procuste

ltinfoprocustecomgt

Para cuencagunizares

Asunto Re analyse de forme chez

les arvicolides

Cabeceras Mostrar todas las

cabeceras

Chegravere collegravegue

Voici le lien pour teacuteleacutecharger le le

setup dinstallation des 3

logiciels APS ApsDigit et ApsUtil

httpwwwprocustecomprogramsset

up_aps_allexe

Noubliez pas de citer APS dans vos

publications

Bien cordialement

Xavier Penin

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 32: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Paacuteginas web recomendadas

bull httplifebiosunysbedumorph la mejor paacutegina de

morfometriacutea geomeacutetrica Contiene un listado con todos los

programas de morfometriacutea Universidad de Stony Brook NY

bull httpwwwmorphometricsorgindexhtml es la paacutegina en la

que se mantiene la lista de email morphmet Es la base de la

organizacioacuten de la morfometriacutea en Suny

bull httpwwwflywingsorgukindexhtm iquestporqueacute hay variacioacuten

morfoloacutegica Universidad de Manchester UK

bull httpwwwprocustecomindexhtml anaacutelisis de la forma con

Procruste Aps Xavier Penin Universidad de Paris V

bull Shapehellipy otros ver la paacutegina de Suny

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 33: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Las ldquoseries TPSrdquo Thin-plate spline

(laacuteminas finas de grosor uniforme)

tpsDIG2

Data acquisition

bull Digitize landmarks amp outlines from image files scanner or video Windows TpsDig is a Windows (95 through XP) program for digitizing landmarks and outlines for geometric morphometric analyses Includes simple image enhancement operations scale factors image brightness profile and support for AVI and MOV files Output is in the TPS file format used by the tps series of programs The TPS file includes the name of the image file for each specimen so that landmarks can always be visualized on the image Since output files are ASCII they can be converted to formats for other software Users should note that the tpsUtil program is useful for both the preparation of the input files to tpsDig as well as for some useful conversions afterwards The program also computes areas of enclosed regions perimeters and linear distances The tpsDig2 link at the left is for the new 205 version

bull Execute the downloaded file to install the program By F James Rohlf

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 34: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Las ldquoseries TPSrdquo

ordenar los archivos digitalizados

TPS util

bull tps file utility program Windows At present it can randomly scramble the order of specimens in a tps file to minimize bias in digitizing landmark locations restore the original order (eg before statistical analyses are performed) create an empty TPS file from a directory with image files add keywords and values to the variables field of a TPS file combine TPS files into a single TPS file deletereorder landmarks remove outlines from a TPS file and convert TPS and NTS files to NTS M or CSV files It can also split an NTS file into several matrices Graphic editors are included for drawing link and sliders files Unbending a deformed specimen Converting outline and curves points to landmarks

bull Other operations can be added - let me know what you need Execute the TPSUTILW32EXE file to install the program By F James Rohlf Version 138

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 35: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

tpsTree

bull Fit and visualize thin-plate splines on trees Windows Performs a least-squares orthogonal generalized Procrustes analysis to obtain a reference and then estimates the splines for each group (internal node) on a tree Both ultrametric (eg WPGMA and UPGMA trees) and additive trees (using squared change parsimony to estimate the shapes of the HTUs) are supported The estimated shapes can be visualized with estimated images in the background (see the tpsSuper program) The file expands into a Windows setup program and associated files By F James Rohlf Version 118 File containing HTU coordinates and HTU images to demo that feature The older and more limited 16 bit Windows version is still available

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 36: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

tpsRegr

bull Multivariate multiple regression of shape onto independent variables Windows performs a multivariate multiple regression of shape (as captured by partial warp scores and the uniform shape component) onto one or more independent variables By appropriate setting up of independent variables this program can perform various types of MANOVA and MANCOVA Can be used to study allometry By F James Rohlf Version 131

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 37: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

tpsSplin

bull Thin-plate spline Windows Program to

compare pairs of specimens by displaying

a Darcy Thompson style transformation

grid based on a thin-plate spline Several

options are given for sliding

semilandmarks The tpssplnwexe file

expands into a Windows setup program

that will be run automatically By F James

Rohlf Version 120

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 38: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

tpsRelw el anaacutelisis de la

deformacioacuten relativa bull Relative warps analysis Windows Performs a

relative warp (deformacioacuten) analysis This corresponds to a principal components analysis of variation in a sample (relative to bending energy if the alpha parameter is not equal to zero) Displays a plot of relative warp scores and a visualization window that displays the estimated shape for arbitrary points in the ordination Also displays partial warp scores and their visualizations Allows fitting of semilandmark points along curves Version 144 By F James Rohlf

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 39: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

tpsPLS

bull Partial least-squares analysis for shapes Windows Used to explore the covariance between a set of variables and variation in shape or between two shapes It performs a two-block partial least-squares analysis It differs from regression in that both variables and shape are considered equally (one is not considered to be a set of independent variables measured without error) Its use is described in Rohlf and Corti (2000 Systematic Biology 49740-753) By F James Rohlf Version 118

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 40: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

tpsSmall

bull Test for small variation in shape Windows Use to test whether the variation in shape among a set of specimens is too large and thus statistical methods based on the tangent space approximation (eg thin-plate spline methods) cannot be used The program can process both 2D and 3D data files Output files include consensus and aligned specimens By F James Rohlf Version 120

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 41: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

tpsPower

bull Estimate statistical power for comparing shapes Windows Program for estimating statistical power for comparing the mean shapes of two populations Provides methods based on several different approaches Can be used for planning morphometric studies as well as to compare power using different approaches Version 104 By F James Rohlf

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 42: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Morpheus et al bull Morpheus et al is a software package that will provide a

comprehensive cross-platform environment for morphometric data analysis It is available in graphical and command-line versions for a number of operating systems (see below) More information about new features and previous changes can be found in the release notes

httplifebiosunysbedumorphmorpheus

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 43: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Multivariate analysis

bull PAST is a free easy-to-use data analysis package aimed at paleontology Windows 95+ Multivariate statistical methods include Principal Components (with Minimal Spanning Tree) Principal Coordinates (ten distance measures) Non-metric Multidimensional Scaling (ten distance measures) Detrended Correspondence Analysis Cluster analysis (three algorithms 11 distance measures) k-means clustering seriation discriminant analysis one-way MANOVA Hotellings t-squared Boxs M Canonical Variates Analysis Also includes Fourier shape analysis thin-plate splines parsimony and time series analysis among others Oslashyvind Hammer University of Oslo

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 44: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Analysis of outlines

bull Shape Elliptic Fourier WINDOWS Extracts the contour shape from a full color bitmap image delineates the contour shape with the elliptic Fourier descriptors and performs a principal component analysis for summarizing the shape information By Hiroyoshi Iwata For more information see Shape home page tutorial or manual

bull Iwata H and Y Ukai (2002) SHAPE A computer program package for quantitative evaluation of biological shapes based on elliptic Fourier descriptors Journal of Heredity 93 384-385

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 45: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Eigenshape analysis

bull httpwwwnhmacukhosted_sitespaleonetftpftphtml

bull This archive contains a series of programs designed to perform an eigenshape analysis on xy coordinate data from object outlines Routines include xy -gt phi() transformation phi() -gt xy transformation eigenshape analysis and projection of objects into the eigenshape space Two completely worked example analyses and a users guide are also provided El autor es Norman McLeod del BMNH de Londres

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002

Page 46: Morfometría, Morphometrics - aragosaurus.com · TD1. Procustes analisis: ... APS program CP Opisthocoelicaudia . Resultados . APS Fecha: ... Procrustes analysis to obtain a reference

Graphical representation of the four-step morphometric protocol A Quantify raw data

(landmarks recorded on body of cichlid fish) B Remove non-shape variation (landmarks of 412

specimens before and after GPA) C statistical analysis (CVA) and graphical presentation of

results Deformation grids for mean specimen for (right) Eretmodus cyanostictus and (left)

Spathodus erythrodon (magnified by 3X to emphasize shape differences) Data from Ruumlber and

Adams 2001 En Adams Rolhf Slice 2002