PARTICIPANTS

Nando Acerbi, Centro di Studi Sociali, La Spezia, Italy

A. Ulises Acuna, Instituto de Quimica Fisica, Rocasolano, Consejo Superior de Investigationes Cientificas, Serrano 119, Madrid 6, Spain

Giancarlo Agostini, Istituto di Chimica Fisica, Universita di Padova, Padova, Italy

J. Ricardo Alcala, Department of Physics, University of Illinois, 1110 w. Green, Urbana, Illinois 61801, USA

Harcel Ameloot, Department of Biophysics, Limburgs Universitair Centrum, Diepenbeek B-3610, Belgium

Sonia Anderson, Department of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon 97331-6503, USA

Alberto Arcioni, Dipartimento di Chimica Fisica, Universita di Bologna, Viale Risorgimento, 4, 40136 Bologna, Italy

Arduino Arduini, Instituto di Scienze Biochimiche, Universita di Chieti, Chieti, Italy

Haria Luisa Barcellona, Istituo di Biochimica, Universita di Catania, Viale Andrea Doria, 6, 95125 Catania, Italy

Francis Baros, Grapp of U.A. 328 of CNRS, ENSIS-INPL I, Rue Grandville F-54042 Nancy, France

Franco Bassani, Scuola Normale Superiore di Pisa, Piazza dei Cavalieri, Pisa, Italy

Peter H. Bayley, Department of Physical Biochemistry, National Institute for Medical Research, Mill Hill, London NW71AA, United Kingdom

Sara Benci, Istituto di Scienze Fisiche, Universita degli Studi di Parma, Via M. d'Azeglio, 85 Parma, Italy

Hario N.H.S. Berberan Santos, Complexo Interisciplinar, Centro de Quimica Estrutural, Av. Rovisco Pais, 1096 LISBOA, Portugal

Ettore Bismuto, Istituto di Chimica Biologica, Universita degli Studi di Napoli, I Facolta di Medicina, Via Costantinopoli, 16, 80138 Napoli, Italy

Ludwig Brand, Department of Biology, Mudd Hall, 30th and Charles St., Johns Hopkins University, Baltimore, Maryland 21218, USA

447

Jean-Claude Brochoo, L.U.R.E., Universite Paris Sud, Bat. 209 D, 91405 Orsay, France

Augusta Brovelli, Dipartimento di Biochimica, Universita di Pavia, Piazza Botta, 27100 Pavia, Italy

Enrico Bucci, Department of Biological Chemistry, University of Maryland Medical School, 660 W. Redwood St., Baltimore, Maryland 21201, USA

Jan Bus, Department of Structure Analysis, Unilever Research Laboratory, P. O. Box 114, 3130 AC Vlaardingen, The Netherlands

Marcello Cacace, Istituto di Biochimica delle Proteine ed Enzimologia, Via Toiano 6, 80072 Arcofelice, Napoli, Italy

Salvatore Califano, Istituto di Chimica Fisica, Universita di Firenze, Firenze, Italy

Paola Cavatorta, Istituto di Fisica, Universita degli Studi di Parma, Via M. D'Azeglio 85, 43100 Parma, Italy

Lin Y-Q. Chen, Department of Chemistry, University of Chicago, 5735 S. Ellis Avenue, Chicago, Illinois 60637, USA

Parkson Chong, University of Virginia, Department of Biochemistry, Jordan Hall, Room 6-17, Charlottesville, Virgina 22908, USA

Jorge Churchich, Department of Biochemistry, University of Tennessee, Knoxville, Tennessee 37996, USA

Nicole Cittanova, Unite Associe 586 du CNRS, UER Biomedicale des Saints Peres, 45 Rue des Saints Peres, 75270 Paris, France

Koen J. K. Clays, Laboratory of Chemical and Biological Dynamics, Katholieke Universiteit of Leuven, Celestijnenlaan 200 D, B-3030 Heverlee, Belgium

Raffaele Colonna, Istituto di Patologia Generale, Universita di Padova, Via Loredan, 16, 35100 Padova, Italy

Robert Cundall, MRC Radiobiology Unit, Chilton, Didcot, United Kingdom

Giovanna Curatola, Istituto di Biochimica, Facolta di Medicina, Universita di Ancona, Via Ranieri, 60131 Ancona, Italy

Robert Dale, Department of Biophysics and Instrumentation, Paterson Laboratories, Christie Hospital and Holt Radium Institute, M20 9BX Manchester, United Kingdom

Maurice R. Kftink, Department of Chemistry, University of MiSSissippi, University, Missippi 38677, USA

Josef Eisinger, Molecular Biophysics Research Department, Room IC 423, AT&T Bell Laboratories, Murray Hill, New Jersey 07974, USA

Roger P. Ekins, Middlesex Hospital, London, United Kingdom

Yves Engelborghs, Laboratory of Chemical and Biological Dynamics, Katholieke Universiteit of Leuven, Celestijnenlaan 200 D, B-3030 Heverlee, Belgium

448

Romana Fato, Dipartimento di Biochimica, Istituto di Chimica Biologica, Via Irnerio, 48, 40126 Bologna, Italy

M. Bernadetta Ferrari, Istituto di Chimica Biologica, Universita di Parma, Ospedale Maggiore, Viale Gramsci, 14, 43100 Parma, Italy

Alessandro Finazzi Agro, Dipartmento di Medicina Sperimentale e Scienze Biochimiche, II Universita di Roma, Tor Vergata, Via Orazio Raimondo, Roma, Italy

Rosamaria Fiorini, Istituto di Biochimica, Facolta di Medicina, Universita degli Studi di Ancona, Via Ranieri, 60131 Ancona, Italy

Nora Folena, Istituto di Pato1ogia Genera1e, Universita degli Studi di Padova, Via Loredan, 16, 35100 Padova, Italy

P. A. George Fortes, Department of Biology, University of California at San Diego, La Jolla, California 92093, USA

Robert Geonis, Department of Biochemistry, University of Illinois, 1209 W. California, Urbana, Illinois 61801, USA

Giorgio Giacometti, Cattedra di Chimica Biologica, Facolta di Scienze, Padova, Italy

Enrico Gratton, Department of Physics, University of Illinois, 1110 W. Green, Urbana, Illinois 61801, USA

Settimio Grimaldi, Istituto di Medicina, Sperimentale del CNR, II Clinica Medica Po1iclinico Umberto I, Roma, Italy

Carlo Giunta, Dipartimento di Biologia Animale, Universita degli Studi di Torino, Via Accademia Albertina, 17, 10123 Torino, Italy

Robert Ball, Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 22709, USA

Myun K. Ban, The Johns Hopkins University, Department of Biology, Mudd Hall, Baltimore, Maryland 21205, USA

Albin H. Hermetter, Institut fur Biochemie, Technical University Graz, Sch10gelstrasse 9, A-8010 Graz, Austria

Jim Herron, Department of Bioengineering, University of Utah, Salt Lake City, Utah 84112, USA

Guy Herve, Institute of Enzymology, CNRS, 91190 Gif-Sur-Yvette, France

Gaetano Irace, Istituto di Chimica e Chimica Biologica, I Facolta di Medicina Universita di Napoli, Via Constantinopoli, 16, 80138 Napoli, Italy

Tze C. Jao, Texaco Research Center, Texaco, Inc., P. O. Box 509, Beacon, New York 12508, USA

Ken Jacobson, Laboratories for Cell Biology, Department of Anatomy, Chapel Hill, North Carolina 27514, USA

David Jameson, Department of Pharmacology, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235, USA

449

Ruud Kraayenhof, Department of Molecular and Cellular Biology, Biological Laboratory, Vrije Universiteit de Boelelaan 1087, 1081 HV Amsterdam, The Netherlands

Ann Kusterdeck, Code 6190, Naval Research Laboratory, Washington D.C. 20375, USA

Sherwin S. Lehrer, Muscle Research Department, Biomedical Research Institute, 20 Staniford St., Boston, Massachusetts 02114, USA

Giampaolo Littarru, Istituto di Biochemica, Facolta di Medicina, Universita degli Studi di Ancona, 60131 Ancona, Italy

James Longworth, Department of Physics, Illinois Institute of Technology, Chicago, Illinois 60616, USA

Giulio Lupidi, Dipartimento di Biologia Cellulare, Universita di Camerino, Camerino, Italy

Henri Magdelenat, Laboratoire de Radiopathologie, Institut Curie, 26 Rue d'Ulm, 75231 Paris, France

William W. Mantulin, Laboratory for Fluorescence Dynamics, Physics Department, University of Illinois, Urbana, Illinois 61801, USA

Gerard Marriott, Department of Biochemistry, University of Illinois, Urbana, Illinois 61801, USA

Giuseppe Ettore Martorana, Istituto di Chimica Biologica, Universita Cattolica, Largo F. Vito 1, 00168 Roma, Italy

Lanfranco Masotti, Istituto di Chimica Biologica, Facolta di Medicina, Universita di Parma, Parma, Italy

Giampiero Kei, Dipartimento di Scienze Biochimiche, Universita di Roma, La Sapienza, Roma, Italy

Maria Da Graca Miguel, Department of Chemistry, University of Coimbra, 3049 Coimbra, Portugal

Roberto Morelli, Istituto di Chimica Fisica, Universita di Milano, Via C. Golgi, 19, 20133 Milano, Italy

Christopher C. Morgan, Department of Biological Sciences, Salford University, Salford MS 4WT, United Kingdom

Andrea Mosca, Dipartimento di Scienze e Tecnologie Biomediche, Universita di Milano, Via Olgettina, 60, 20132 Milano, Italy

Guido Motolese, I.S.S. s.r.l., Ceparana La Spezia, Italy

Alejandro Paladini, INGEBI, Obligado 2490, 1428 Buenos Aires, Argentina

Tiziana Parasassi, Dipartimento di Chimica, Istituto di Medicina Sperimentale del CNR, Piazzale Aldo Moro, 5, 00185 Roma, Italy

Francesco Pennisi, Cremascoli S.p.a., Via C. Prudenzio, Milano, Italy

Licinio C. Pereira, Universidade do Minho, Largo do Paco, 4719 Braga, Portugal

450

Hario Piacentini. Istituto Struttura della Materia del CNR. Frascati (Roma). Italy

David Piston. Department of Physics. University of Illinois. Urbana. Illinois 61801. USA

Deleana Pozzi. Dipartimento di Medicina Sperimenta1e. Po1ic1inico Umberto I. Roma. Italy

Gregory Reinhart. Chemistry Department. University of Oklahoma. Norman. Oklahoma 73019. USA

Renato Angelo Ricci. Dipartimento di Fisica. Universita di Padova. Padova. Italy

Nicola Rosato. Centro di Bio10gia Mo1eco1are del CNR. c/o Dipartimento di Scienze Biochimiche. Universita di Roma. La Sapienza. Roma. Italy

Jane Rosen. 180 Thompson St .• Apt. 6D. New York. New York 10012. USA

Catherine Royer. Laboratoire de Bio10gie Physico-Chimique. Paris VII -CNRS. 10 rue des P1antes. 91230 Montgeron. France

Gaetano Saitta. Istituto di Struttura della Materia. Universita di Messina. Via dei Verde. Messina. Italy

Gautam Sanyal. Department of Chemistry. Hamilton College. Clinton. New York 13323. USA

Keir Shinitzky. Department of Membrane Research. Heizmann Institute of Science. Rehovot. Israel

Jerson Silvia. Department of Biochemistry. University of Illinois. Urbana. Illinois 61801. USA

Peter St.s. Thrombosis/Hematology Program. OMRF. 825 N.E. 13th St •• Oklahoma City. Oklahoma 73104. USA

Angelo Spinedi. Dipartimento di Bio10gia. II Universita di Roma. Tor Vergata. Via o. Raimondo. 173. 00173 Roma. Italy

Arthur G. Szabo. Department of Biological Sciences. National Research Council. 100 Sussex Dr •• Ottawa. K1AOR6 Canada

Patrick Tauc. Institute d'Enzymo10gie du CNRS. Gif-Sur-Yvette. France

Richard Tba.pson. Code 6190. Naval Research Laboratory. Washington D.C. 20375-5000. USA

Bernard Valeur. Conservatoire National des Arts et Metiers. Chimie General. 292 Rue Saint-Martin. 75141 Paris. France

Arnaldo Vecli. Dipartimento di Fisica. Universita deg1i Studi di Parma. Via M. D'Azeg1io. 85. 43100. Parma. Italy

Paola Viani. Dipartimento di Chimica e Biochimica Medica. Faco1ta di Medicina Universita di Milano. Via Sa1dini. SO. 20133 Milano. Italy

Kiebel Vincent. Department of Biochemistry and Biophysics. ER 64-01 CNRS. 45 rue des Saints-Peres. Paris. France

451

A.J.W.G. Visser, Agricultural University, 6703 BC Wageningen, The Netherlands

Edward Voss, Jr., Department of Microbiology, 131 Burril Hall, 407 S. Goodwin St., University of Illinois, Urbana, Illinois 61801, USA

Therese Wiedmer, Thrombosis/Hematology Program, OMRF, 825 N.E. 13th St., Oklahoma City, Oklahoma 73104, USA

Gregorio Weber, Department of Biochemistry, University of Illinois, 1209 W. California St., Urbana, Illinois 61801, USA

Claudio Zannoni, Istituto di Chimica Fisica, Universita di Bologna, Viale Risorgimento, 4, Bologna, Italy

Lello Zolla, Dipartimento di Bologia Cellulare, Universita di Camerino, 62032 Camerino, Macerata, Italy

452

AUTHOR INDEX

Acuna, A. U., 353 Alcala, J. R., 17 Alpert, B., 429 Ameloot, M., 430 Anderson, S. R., 217 Anfinsen, C., 360 Arcioni, A., 393 Arduini, A., 433

Bar, L., 374 Barenholz, Y., 374 Bassani, F., 1 Bassler, B., 369 Bayley, P., 389 Belfiglio, M., 433 Berni, R., 87 Bertinelli, F., 393 Brand, L., 33, 360, Brochon, J. C., 361, Bucci, E., 305 Burrows, H. D., 440

369 436

Cannella, C., 87 Castelli, A., 414 Cavatorta, P., 173, 383 Chen, L. X.-Q., 432 Chiancone, E., 411 Chong, P. L.-G., 374 Christeff, N., 434 Churchich, J., 419 Cidlowski, J. A., 419 Cittanova, N., 434 Clays, K., 378 Conti, F., 432

Damonti, V., 433 De Cesare, M., 433 Dejulia, J., 433 Desfosses, B., 434 De Smedt, H., 430 de Wolf, F. A., 366 DiBartolomeo, A., 433 DiGuiseppi, J., 139

Eftink, M. R., 437 Eis~nger, J., 151 Engelborghs, Y., 378, 381

Farruggia, G., 173

Finazzi Agro, A. , 87, Fleming, G. R. , 432 Forosinho, S. J. , 440 Fronticelli, C. , 305

Gallay, J. , 397 Ghiron, C. A. , 437 Gratton, E. , 17, 375,

432 Gryczynski, I. , 305

Han, M. K. , 33, 360 Hazlett, T. L. , 61 Hendricks, H. , 430 Hermetter, A. , 357 Herron, J. N. , 364 Herve, G. , 436 Holifield, B. , 139

Ishihara, A., 139 Ishii, Y., 423

341

411,

Jacobson, K., 139 Jameson, D. M., 61, 364 Jao, T. -C., 435

Knutson, J. R., 33, 360 Kraayenhof, R., 366 Krab, K., 366 Kranz, D. M., 364 Krenz, K. L., 435 Kusterbeck, A. W., 426

Lakowicz, J. R., 305 Lehrer, S. S., 423 Lessick, R., 360 Lillo, M. A., 353 Livesey, A. K., 361

Malak, H., 305 Malencik, D. A., 217 Mancinelli, G., 433 Mantulin, W. W., 115 Martin, S., 389 Martorana, G. E., 414 Masotti, L., 173, 383 Meucci, E., 414 Miggiano, G.A.D., 414 Miguel, M. G., 440

453

Mordente, A., 414

Neyroz, P., 33, 369 Nicot, C., 397

Pa1adini, A. A., 101 Paltauf, F., 357 Parasassi, T., 432 Persoons, A., 378 Piacentini, M., 91 Prendergast, F. G., 17,

401

Quagliariel10, E., 383

Rainer, B., 357 Rajkowski, K. M., 434 Riccio, P., 383 Reinhart, G. D., 195 Rosato, N., 87, 411 Roscher, A., 357 Roseman, S., 369 Royer, C., 429, 436

Sang, H.W.W.F., 366 Santini, S. A., 414 Sanyal, G., 401 Sapora, 0., 432 Sartor, G., 173

454

Schwabe, G., 357 Shinitzky, M., 133 Silva, J. L., 101 Stefanini, S., 411 Sterk, G. J., 366 Stern, A., 433 Szabo, A. G., 173, 383, 406

Tanoni, R., 393 Tanc, P., 436 Thompson, R. B., 375, 426 Thompson, T. E., 374

Valeur, B., 269 VandeVen, M., 360 van Walraven, H. S., 366 Vincent, M., 397 Visser, A., 319 Voss, E. W., Jr., 247, 364

Waks, M., 397 Walbridge, D. G., 33, 360 Wasylewski, Z., 437 Weber, G., 101, 343

Yamashita, S., 406

Zannoni, C., 393

SUBJECT INDEX

ACTH. 218. 397 Activation volume. 126 Active site modifications. 87 ADONE storage ring. 91 Alcohol dehydrogenase

acid denaturation. 38 intrinsic fluorescence. 38. 369. 437

Alkaline phosphatase. 414 Alkarylsulfonates. 435 Allosteric behavior. 203 Aminoacyl-tRNA (aatRNA)

ethidium bromide complex. 78 fluorescein labeled. 78 ternary complex. 62

Anisotropy decay. 399. 421. 435 decay associated spectra (ADAS). 35 steady-state. 154. 179. 219. 292. 353. 357. 402. 430 time-dependent. 73. 327

Antibodies anti-fluorescyl. 247. 363 anti-GP80 (rhodamine conjugated). 145 anti-rat IgG (rhodamine conjugated). 147

Apohemoglobin. 429 Apomyoglobin. 429 Argon-ion laser. 175. 307. 321 ATCase. 363. 436 Azurin. 25

Bacteriorhodopsin. 154 Bichromophore. 269 Bombesin. 173

complex with DMPS. 174 complex with lysolecithin. 174

Bovine Serum Albumin (BSA). 105 Broccoli. 133 Brownian motion. 151

Calcium. 143. 217 Calmodulin. 217. 401

binding to MLCK. 223. dansyl derivative. 222 dityrosine formation. 233 melittin binding. 226

Calmodulin Binding Peptides. 225 CBP1. 401

455

456

CBP2, 401 CCD, 141 Cell differentiation, 442 Cell locomotion, 145 Chirality, 133 Chiron, 138 Chi-square (X 2 ), 39, 70, 89, 181, 311 Cholesterol, 433 Circular Dichroism, 136, 176, 229, 254, 383, 399, 401 Colchicine, 381 Color centers, 12, 91 Conformational States, 261 Continuous lifetime distribution models, 17, 70, 389, 403, 411 Coupling enthalpy, 211 Coupling entropy, 211 Coupling free energy, 197 Correlation matrix, 181 Critical transfer distance, 272, 346 Cytochrome b-c1, 162 Cytoskeleton, 433

Debye rotational relaxation time, 68 Debye-Sears tank, 69 Decay-associated spectra (DAS), 33 Dehydroergosterol, 374 Deibler-MBP, 383 Dexter's theory, 274 Diffusion

Fick's law, 155 in membranes, 144, 151 lateral, 118 mean-time-to-capture, 167 obstructed, 162 rotational, 154, 345 translational, 154, 274, 345

Diffusive transport, 166 Digitized Fluorescence Microscopy (DFM), 139 Dipole-dipole interaction, 272 Distribution of distances, 296 Distribution of rate constants, 296 Dityrosine, 233 Durbin-Watson parameter, 330, 390 Dynamic averaging, 273 Dynamic polarization equations, 72

Einstein coefficients, 3 Electrophoresis (slab gel), 101 Elongation factor Ts (EF-Ts), 65 Elongation factor Tu (EF-Tu), 61

intrinsic fluorescence, 64 polarization, 68 ternary complex, 62 time-resolved fluorescence, 71

Enantiomers, 135 ~-Endorphin, 218 Energy transfer, 88, 253, 269, 314, 330, 345, 426 Equilibrium sink, 264 Erythrocytes, 153, 433 Estriol, 434 Eximer, 122, 269, 423

Eximer membrane probes, 155 Exiplex, 269

Fab fragments, 259 Fermi-Dirac particles, 137 Fermi golden rule, 6 Ferritin, 411 Fibrinogen (dansylated), 355 Fibroblasts, 145, 357 Flavins, 61, 288, 319, 327, 343 Flavodoxins, 334 Flavoproteins, 328, 343 Fluidity microscopy, 152 Fluorescence Probes

N-acetyltryptophanamide (NATA), 175 N-(3-sulfopropy1) acridinium, 377 ANS, 64 anthracene, 279 anthrone, 281 9-anthroylcholine, 223 bilirubin, 289 carbazole, 276 coumarin, 290 dansyl, 47, 61, 109, 222, 275, 356 dimethyl POPOP, 277 DODCI, 379 DPH, 127, 357, 358, 393, 430, 433, 442 erythrosin B, 325 ethidium bromide, 62 excimer membrane probes, 155 FAD, 319 FITC, 255, 426 FMN, 327 fluorene, 282 fluorescamine, 61 fluorescamine-GDP, 75 fluorescamine-GTP, 78 fluorescein, 61, 248 Fura-2, 143 lAEDANS, 363 indole, 277, 406 indole alkaloids, 281 lucifer yellow, 434 malachite green, 379 merocyanin-540, 433 l(N)-methylindole, 281, 406 naphthalene, 276 NBD-PC, 121 phenol, 277 phthalimide, 276 porphyrin, 288, 429 p-terphenyl, 277 pyrene, 42, 122, 152 pyrene-~-mercaptoethanol, 423 pyrene butyric acid, 200 pyrene dodecanoic acid, 159 pyrene DTT, 423 pyrene maleimide, 423 pyridoxal-5-phosphate, 109, 419 riboflavin, 320

457

RITe, 426 rhodamine antibody conjugates, 145, 147 rhodamine B sulfonyl-PE, 121 tetramethylrhodamine isocyanate, 426 TMA-DPH, 357, 430 trans-stilbene, 278 trintrobenzene sulfonic acid, 433 uranyl ion, 440

Fluorescence Recovery After Photobleaching (FRAP), 144, 151 Forster's theory, 272, 346 Fourier Transform (image), 144 Free energy diagram, 196 Frequency domain fluorometry, 306 Gaba-transaminase, 419 Global analysis, 34, 182, 360 Glucagon, 218, 398 Glutathione reductase, 329 GP80, 143 Guanosine nucleotides, 62

Hamiltonian, 6 Harmonic content, 23, 70, 95, 378 Hemoglobin, 305, 433 High pressure cell, 102 Hydrophobic Interactions, 425 Hydrostatic pressure, 101

effect on ionization constants, 108 fluorescence polarization, 103 perturbation of lipid transfer, 125 protein dissociation, 101 slab gel electrophoresis, 101

Idiotype, 250 Immunoassay, 426, 434 Immunofluorescence, 139 Intramolecular

electron transfer, 270 excimer formation, 269 exciplex formation, 269 excitation energy transfer, 270 photochemical reaction, 270

Intrinsic protein fluorescence dityrosine, 233 tryptophan, 20, 39, 45, 50, 64, 87, 173, 218, 360, 369, 381, 383, 397,

401, 411, 432, 436 tyrosinate, 24 tyrosine, 65, 234

Inverted(reversed) micelles, 398, 435 ISIT tubes, 141 Isotypy, 247

Lapis solaris, 1 Lifetime heterogeneity analysis

distribution, 17, 71, 442 global analysis, 34 maximum entropy method, 361 method of moments, 34 non-linear least squares, 24, 34, 70, 175

458

Linkage, between multiple ligands, 203 and dynamics of rat liver PFK, 210 and PFK aggregation, 198 thermodynamic, 195

Lipid structural order parameter, 433 Lipid transfer, 116 Liquid crystal (thermotropic), 393 Lysoamide dehydrogenase, 330

Marquard algorithm, 175, 379, 389 Membranes

biological, 357, 433, 440, 442 brush border, 430 fluid mosaic model, 151 fluidity, 127, 151, 357, 433 phase-state asymmetry, 433 water interface, 358

Metalloprotease, 437 Micellization, 440 Microchannel plates, 52, 141, 175, 308 Microemulsions (w/o), 440 Microscopy

DFM instrument, 140 digitized fluorescence (DFM), 139 fluidity, 152 fluidity imaging, 168 FRAP, 144, 151 video FRAP, 144

Milling crowd model, 157 Mode-locked lasers, 23, 35, 69, 307, 378 Monoclonal antibody, 247 Multifrequency phase and modulation fluorometry, 12, 69, 94, 279, 376,

378, 381, 403, 426, 437, 442 Multistate models, 263 Myelin basic protein, 383 Myoglobin, 18, 105

Nd:YAG laser, 36 Non-linear least squares analysis, 353, 360 Non-radiative transfer, 271

Optical rotation, 135 Order parameter, 430 Orientation factor, 273 Overlap integral, 273, 281

Parity, 133 Peptides

amphiphilic, 401 bombesin, 173 crabrolin, 227 dermorphin, 173 dynorphin, 229 helodermin, 227 hormones, 397 mastoparan polistes, 218, 401 mastoparan x, 222, 401 melittin, 226, 401 sauvagine, 173

459

460

Percolation theory, 162 Perrin equation, 68, 154 Perrin (Perrin-Weber) plot, 201, 353 Phase-resolved spectra, 438 Phenylhydrazine, 433 Phosphoenolpyruvate: glucose phosphotransferase (PTS)

DTNB modified, 46 enzyme I, 41 enzyme II, 42 HPr, 41 pyrene maleimide conjugate, 42 quenching, 369

Phosphofructokinase (PFK), 195 aggregation of, 198

Phospholipase A2, 25, 433 Plasma lipoproteins, 116

high density (HDL), 117 low density (LDL), 117 very low density (VLDL), 117

Plasmologens, 357 Pockels' cell, 69 Poisson noise, 38 Polaritons, 14 Polarization, 68, 103, 200, 292, 311, 323

dynamic, 393, 403 excitation spectra, 419 steady-state, 292

Polyethylene glycol (PEG), 257 Proerythroblasts, 442 Protein conformational change, 369, 381 Protein dynamics, 17, 210, 435 Protein phosphorylation, 230 Pulse fluorometry (time-correlated single photon counting), 35, 175

Quantum theory, 5 Quantum Yield, 178, 248, 344, 402 Quenching, 87, 219, 252, 305, 320, 346, 369, 381

acrylamide, 67, 372, 285, 403 cesium, 372 iodide, 67, 369, 381, 403 oxygen, 403

Radiative transfer, 271 Rhodanese, 87 Riboflavin, 319, 343 Ribulose-5-phosphate kinase, 437 RNase T1, 25, 432, 437 Rotational correlation time, 353, 360, 374 Rotational diffusion, 423 Rotational relaxation time, 435

Scatchard plot, 180 Scintillators, 271, 278 Scorpion neurotoxin variant 3 (NTV3), 25 Shannon-Jaynes entropy function, 361 SIT tubes, 141 Sodium bis(2-ethylhexyl) sulfosuccinate (AOT), 398 Sodium fluoride crystals, 91 Solar concentrators, 271 Soleillet products, 353 Solvent perturbation, 254

Solvent reorientation, 406 Species associated spectra (SAS), 34 Spectrin, 433 Spin-label (guanosine nucleotide analog), 77 Spontaneous lipid transfer, 118, 374 Standard volume change, 364 Static isotropic averaging, 273 Stern-Volmer equation, 67, 377, 380, 403 Stokes-Einstein law, 432 Stokes shift, 2 Stopped-flow (fluorescence), 381 Structure-function models, 256 Subunit association, 369 Succinic semialdehyde dehydrogenase, 419 Synchrotron radiation, 69, 91, 399

Temperature-jump, 261 Testosterone, 434 Thioredoxin, 50, 360 Time-resolved emission spectra (TRES), 33, 187 Time-resolved instrumentation, 23, 36, 69, 95, 175, 307, 321 Transfer depolarization factor, 279 Transfer efficiency, 273 Translocases, 115 Triplett state, 348 Tropomyosin, 423 Tryptophan Synthase, 107 Tubulin, 381 Two-state models, 262

Van1t Hoff analysis, plots, 211, 252, 364 Viscosity, 294, 355, 423

WSSR, 323

Yeast 3-phosphoglycerate kinase, 437

Zellweger syndrome, 357

461