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REPORTERS:Artuz, Jonesa Jay
Pueblo, John Alfrey D.Sael, Chellamae A.Saleh, Shahina S.
Quijano, Quinee T.
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BEFORE AFTER
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ObjectiveIntroduction
ContentsRequirements for the accredition of water analysislaboratoriesMinimum equipments for each service.Minimun required refference for laboratory.SynthesisEvaluation
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After completion of this lesson our classmateswill be able to:
explain the importance of water testinglaboratories.identify the standard laboratory proceduresin the examination of drinking water.enumerate the requirements foraccreditation of water analysis laboratories.
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-PD 866 (Code of sanitation of Philippines)
-Examination of drinking water ---Department of Health.
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January 1968 (Ministry of HumanSettlements and Ecology)
-published a three- volume PhilippineStandards Methods for Air and WaterAnalysis September 1978 (Ministry of Health)-issued a revised National Standards forDrinking Water
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-assurance that accepted procedures
available for determination of STANDARD
-adherence to the procedures allowscomparability of results of analysis within asingle laboratory and between laboratories.
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Water analysis laboratories maybeaccredited for separate services like:
BacteriologicalChemicalRadiologicalBiological
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Name, citizenship and domicile of the head of the laboratoryMunicipality and where it is establishedName of establishmentName, citizenship and domicile of ownerScope and nature of work, specifying procedures
Statement that applicant has complied with allbusiness requirements
Tax clearance for proceeding year
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sanitary engineer .In areas where there may not be a licensed
sanitary engineer,licensed chemistmedical technologist
pharmacist
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headmanageor supervise up to 3 waterlaboratories
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technical aspect:physical bacteriological,
chemical,biological and radiological examinations of water ,
should be a registered: pharmacist,medical technologist,or chemist.
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must have been trained and musthave at least 1 year experience.
may supervise not more than two
skilled workers .
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Laboratory support personnelsuch as;laboratory aides - are needed to clean glassware's, assist in thepreparation of media.
Each service must therefore be operated by at least oneregistered professional, one laboratory aide .
Bacteriological service-----handle at most 30 samples/day
Chemical service -----5 samples for routine chemistry .
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Work rooms must be:house in a permanent buildingWell-ventilated
correlate with the volume and type of workA bench height of 90 cm providesconvenience for workers
adequate running water.Adequate physical provisions for the safetyof the laboratory personnel must beprovided.
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Quality -to meet the levels of sensitivity,reliability and should only need
minimum service repair.Increased workloadEfficiency and effectively.Highest grade of quality media andreagent are to be used
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A. bacteriological Analysis Service
B. biological Analysis Service
C. Physical and Chemical Analysis Services
D. Radiologic Analysis Service
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Multiple-tube fermentation technique
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IMVIC test Standard plate count
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Fecal coliform test Optional-test for fecal streptococcal group
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ColorOdorPh valueSpecific conductanceTasteTemperatureTurbidityTotal solidsHardness
AcidityAlkalinityAluminumArsenicBariumCadmiumCalcium free CO2ChlorideChlorine (residual)
TEST FOR
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Chromium(hezavalent)CopperCyanideFluorideIronLeadMagnesiumManganeseMercury
Nitrogen, ammoniaNitrogen nitrateOil and greaseTotal organic carbonOxygen, dissolvedOxygen, demand,biochem.PesticidesPhenolsPhospahate
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PotassiumResidueSeleniumSilica, dissolvedSilver
SodiumSulfateSulfideSulfiteSurfactantzinc
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Total radioactive strontium in H2O
Strontium --- 90 in H2OTotal Radium (Precipitation Technique)Radium ---- 266 by Radon (Soluble, suspendedand Total)
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IncubatorWaterbath, 37C45C 0.5(Escherichia coli test is to be performed)
AutoclavepH meterAnalytical balanceColony counter
MicroscopeHot plate/stoveBunsen burnerRefrigerator
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Incubator
Waterbath
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Autoclave
pH meter
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Analytical balance
Hot plate/stove
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Bunsen burner
Refrigerator
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pH meterOven sterilizingCandle turbidimeter
Pipette washerSpectrophotometer, spectronic 20, or AAMagnetic stirrerWaterbathRefrigeratorGas chromatographerVacuum filter
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Oven sterilizing
Candle turbidimeter
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Pipette washer
Spectrophotometer
Magnetic stirrer
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Gas chromatographer Vacuum filter
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1. Water sampler, any of the following:a. Van dornb. Kemmerrerc. Wansend. Buttnere. Improvised Neyer or LLDAs Modifier
Sampler2. Water pump with graduated intake3. Bottom drodge4. Inverted microscope5. Compound microscope
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Bottom dredge
Inverted microscope
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a. Philippine Standard Methods for Air andWater Analysis, vol.1 and vol.2, HumanSettlements Commission, January 1978Standard Methods for the Examination of Water and Wastewater: APHA, AWWA,WFCF, 14th edition.
National Standards for Drinking Water,Bureau of Research and Laboratories,Ministry of Health, 1978.
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In every glass of water we drink, some of the water has already passed through fishes, trees, bacteria,
worms in the soil, and many other organisms,including people...Living systems cleanse water and
make it fit, among other things, for humanconsumption.
ELLIOT A. NORSE
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