CENTENARIO DE LA CREACIÓN DE LOS ESTUDIOS MICROBIOLÓGICOSEN LA UNIVERSIDAD NACIONAL DE LA PLATA

1912 – 2012 V JORNADAS DE MICROBIOLOGIA CLINICA, INDUSTRIAL Y AMBIENTAL

DE LA PROVINCIA DE BUENOS AIRESLa Plata, Prov. de Buenos Aires 8, 9 y 10 de octubre de 2012

Carrera de Microbiología Clínica e Industrial Facultad Ciencias Veterinarias. UNLP.

BIOFILMS EN DIFERENTES INDUSTRIAS. ALTERNATIVAS PARA SU CONTROLGuiamet PatriciaINIFTA, Departamento de Química, Facultad de Ciencias Exactas, UNLP, CCT La Plata - CONICET. CC 16, Suc. 4 (1900), La Plata, Argentina.Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, CONICET. pguiamet@inifta.unlp.edu.ar

Reuniones relevantes ( + de 70)

■ First Workshop Argentine-USA (CONICET-NSF) on Biodeterioration of Materials, INIFTA, La Plata, 1984.

■ Dahlem Conference on the Structure and Functions of Biofilms, Berlin, Germany, 1988.

“Redox/metal dissolution in biofilms” H.A.Videla.

Medio

Metal

Zona anaeróbica

Ánodo

CátodoCátodo pH

Ausencia de O2

Productos corrosivos

" Microbiological Spoilage of Aqueous Based Surface Coatings". (1996).

P.S.Guiamet and H.A.Videla, Corrosion Reviews, Vol. 14, (1-2), p.47-57.

" Activity of isothiozolone biocide against Hormoconis resinae in pure and mixed biofilms". (1996).

P.S.Guiamet and C.C.Gaylarde.World Journal Microbiology & Biotechnology, Vol.12, p. 395-397.

“Fungal contaminants of jet fuels”. (1995).H.A.Videla, P.S.Guiamet, E.H.Reinoso.Biodeterioration & Biodegradation 9 (A. Bousher, N. Chaudne, R. G. Edyvean, ed.), p. 615-620.

Panel de cobre - Panel testigo (sin cubierta de cobre)“Antifouling effects of two satured copper coating applied on carbon

steel structures" (2008)Guiamet P., Gómez de Saravia S.

Revista Metalurgia 44 (5): 398-405 (ISSN 0034-8570)

Understanding biocorrosion in practical cases of different industrial systems

Patricia Guiamet., Paola Lavin, Mónica Zapponi, Sandra Gómez de Saravia

The aims are a) to present several case studies of MIC found in three plants of extraction and secondary oil recovery by water injection, due to operating problems. Plants are located in the provinces of Neuquén (systems 1 and 2) and Mendoza (system 3) b) to suggest possible solutions for these problems.

System 1• SRB concentration up to 100 times higher than the maximum value of 104, generating then H2S.

• The most important problem was connected with the use of a non oxidizing biocide (acrolein). It was started with two patching per week of 1100 ppm, each of them during one hour.• At the end of 2004 a dispersant plus biocide was used. Pressure increased so much that serious damages occurred in the system. • Some 15m3/hr. of fresh water from the Colorado river enter to the water treatment system into the washing tank. This could be a source of nutrients for the SRB.

System 2

• SRB (1015 mo/mL) were obtained. These high values observed in different

systems are frequently due to staff dismissals caused by socioeconomic problems

or to operators working without the suitable training.

•Tetrakishydroxymethyl phosphonium sulphate (THPS) was applied.

There are high H2S concentrations of genetic and microbiological origin.

•The treatment of water to be reinjected is not very effective.

•There are too much solids.

BRS PIAS

0

2

4

6

8

10

12

14

16

Ene Feb Mar Abr May Jun Jul Ago

caldos

Ent. Planta Sda. Planta Pozo Iny, o Colector

System 3 • Blockage of the wells by solids (> 40% per year). The well blockage causes a significant injection pressure increase. • There is a generalized problem of corrosion in the valves. • The processes of the plant are not automatic . It is an operator who regulates the pumps and do other operations manually, so the smallest periods of concentrations is enough to cause problems. • There are high concentrations of sulphides would be deposited on the pipes. • Biocides used were glutaraldehyde, acrolein and others.

ALTERNATIVAS PARA SU CONTROL

• Productos Naturales

• Productos Químicos

Centaurea cyanusCichorium intybus

" Activity of isothiozolone biocide against Hormoconis resinae in pure and mixed biofilms".

(1996).P.S.Guiamet and C.C.Gaylarde.

World Journal Microbiology & Biotechnology, Vol.12, p. 395-397.

“ The antimicrobial activity of an aqueous extract of Brassica nigra “. Gómez de Saravia S. G.and Gaylarde C.C. International Biodeterioration and Biodegradation 41 (1998) 145-148

“An innovative method for preventing biocorrosion through microbial adhesion inhibition”. (1999).P.S.Guiamet, S.G.Gomez de Saravia and H.A.Videla. International Biodeterioration & Biodegradation,43 (1-2), 31-35.

Global solution proposal for problems of the systems 1, 2 and 3 ∗ Service of microbiological management of the systems and a set of consultancies and specific services. ∗ To collect, organize and display all the information related with microorganisms, as a tool for monitoring, controlling and preventing the bacteria presence in the oil systems. ∗ To train the operators and responsible staff of the plants in order to decrease at the maximum operational mistakes∗ Theoretical and practical courses on biofouling, biofilms and biocorrosion issues will be provided to the operators.∗ Collecting qualitative-quantitative data from planktonic and sessile bacteria producing biofilms of the systems.∗ Monitoring through sampling devices∗ To propose the use of the suitable biocides according to studies performed by authorized staff.

ConclusionesConclusionesBacterias heterotróficas

mesófilas aeróbicasBacterias productoras de ácidoBacterias sulfato reductorasBacterias reductoras de sulfito

Potencial riesgo de biocorrosión

Bajos valores de pH

Altas concentraciones de cloruros y sulfatos en el lixiviado

Riesgo de corrosión inorgánica

Plan de seguimiento a largo Plan de seguimiento a largo plazo…plazo…

Estudios fisicoquímicos

Estudios microbiológicos

EVITAR PARADAS DE PLANTA, RECAMBIO DE PIEZAS Y PÉRDIDAS

ECONÓMICAS SIGNIFICATIVAS

Equipo

• Dra. Patricia Guiamet• Dra. Sandra Gómez

de Saravia• Lic. Patricia Battistoni• Lic. Paola Lavin