MariaGuadalupeMartinez,Ph.D.www.linkedin.com/in/mguamartinez

Address:327East92ndSt.Apt4B.NY10128.Phone:(+1)917-902-2187.Email:mguamartinez@gmail.com

Education2010 Ph.D.inBiochemistry.UniversityofBuenosAires,Argentina.2005 Bachelor in Molecular Biology and Biotechnology. April 2005. University of Buenos Aires,

Argentina.ProfessionalexperienceAlbertEinsteinCollegeofMedicine,NewYork,US. 2010-presentSeptember2015-present ResearchAssociate,DepartmentofCellBiology September2010-September2015 ResearchFellow,DepartmentofCellBiology UniversityofBuenosAires,BuenosAires,Argentina. 2005-2010April2005-August2010 Ph.D.student,DepartmentofBiochemistry April2004-April2010 Intern,DepartmentofBiochemistry Scientific/technicalskills• Molecularbiology: cloning. PCR,RT-PCR,mutagenicPCR,DNA sequencinganalysis, plasmiddesign

andconstruction.Viral transduction.Mammaliancellselectroporation. Immunoprecipitation.Pulse-chase assays using 35S DNA extraction. RNA synthesis. RNA transfection/electroporation inmammaliancells.SDS-PAGEwesternblot,ELISA,kinaseassays.

• Virology:manipulationofpositiveBSL-2(+)pathogens:ExperienceworkingwithemergingpathogenssuchasChikungunya,DengueandZikavirus.Design,productionandcharacterizationofalphavirusmutantsusinginfectiousclones.

• Cell Biology: Construction andmaintenance of stable and inducible stable cell lines, FACS samplepreparation. Manipulation of neural cell lines, including neural progenitor cells derived frominduciblepluripotentstemcells.ProteinknockdownbydominantnegativeexpressionorsiRNA.

• Microscopy: Confocal: Fix and live cell imaging and FRAP. Total internal Reflection FluorescenceMicroscopy:Liveandfixcell imaging.LightMicroscopy: livecell imaging.Brightfield,phasecontrastandDIC.Transmissionandscanningelectronmicroscopy.Correlativelightandelectronmicroscopy.Superresolutionmicroscopy:STORMandSIMsamplepreparation.

Non-laboratoryscientificskills• Teamleadership:mentoredandmanagedstudentsandpost-docsinthelabindifferentscientific

projects.• Imagingsoftware:Volocity,Imaris,ImageJ,ImageQuant

• Programminglanguages:§ 2015CertificateinRProgramming,Coursera/JohnsHopkinsUniversity§ 2015CertificateinProgrammingforeverybody,Coursera/MichiganUniversity

• Computer:Microsoftoffice,AdobePhotoshopandillustrator,endnote,Genious,Lasergene,vectorNTI,Prism.

Publications1. “Intercellular Extensions Are Induced by the Alphavirus Structural Proteins and Mediate Virus

Transmission ”Martinez MG & Kielian M (2016) “Intercellular Extensions Are Induced by theAlphavirusStructuralProteinsandMediateVirusTransmission”.PLoSPathog12(12):e1006061.

2. "EntrystudiesofNewWorldarenaviruses".MartinezM.G.,ForlenzaM.B.,CandurraN.A.,Cordo,S.M.Manuscriptsubmittedforpublication.October2016.

3. “Human transferrin receptor triggersanalternativeTacaribevirus internalizationpathway”Roldán,J.S.,MartinezM.G.,ForlenzaM.B.,WhittakerG.R.andCandurraN.A.ArchVirol.2015.

4. “Imaging theAlphavirus Exit Pathway”MartinezM.G., Snapp E.L., PerumalG.S.,Macaluso F.P andKielianM.J.Virol.2014.Coverarticle.

5. “Utilization of humanDC-SIGN and L-SIGN for entry and infection of host cells by theNewWorldarenavirus,Junínvirus”.MartinezM.G.*,BialeckiM.A.*,BelouzardS.,CordoS.M.,CandurraN.A.andWhittakerG.R.BiochemBiophysResCommun.2013.

6. “Membrane localization of Junín virus glycoproteins requires cholesterol and cholesterol richmembranes”.CordoS.,ValkoA.,MartinezM.G.,CandurraN.BiochemBiophysResCommun.2012.

7. “S-layer proteins of Lactobacillus acidophilus Inhibitory effect of JUNV infection”.MartinezM.G.*,PradoAcostaM.*,CandurraN.A.andRuzalS.R.BiochemBiophysResCommun.2012.

8. "Anantibodyrecognizingtheapicaldomainofhumantransferrinreceptor1efficientlyneutralizesallNew World hemorrhagic fever arenaviruses" Helguera G., Jemielity S., Abraham J., Cordo, S.,MartinezM.G.,RodríguezJ.,BregniC.,WangJ.,FarzanM.,PenichetM.,CandurraN.A.andChoeH.JVirol.2012

9. “Involvement of cellular proteins in Junin arenavirus entry” Martinez M.G., Forlenza M.B. andCandurraN.A.ShortCommunicationBiotechnologyJournal.2009.

10. “InvolvementofcytoskeletoninJuninvirusentry”MartinezM.G.,CordoS.andCandurraN.A.VirusRes.2008.

11. “CharacterizationofJunínarenaviruscellentry”MartinezM.G.,CordoS.andCandurraN.A.JournalofGeneralVirology.2007.

ParticipationinscientificmeetingsInvitedtalksandtalksselectedfromabstracts• 2016. KeystoneSymposia: “Positive- StrandRNAViruses.”Austin, TexasUSA.Short Talk andposterpresentation:“Alphavirusbudding:howvirusesremodelthecellduringexit”MartinezM.G.&KielianM.

• 2015. Invited speaker. Microscopy and microanalysis meeting. “Imaging alphavirus exit pathway”.MartinezM.G.Portland.US.

• 2015.Invitedspeaker.ArgentinianConferenceofVirology.“Alphavirusbudding:howvirusesremodelthecellduringexit”.MartinezM.G.BuenosAires,Argentina.

• 2014. Keystone Symposia: “The Ins and Outs of Viral Infection: Entry, Assembly, Exit and Spread.”Breckenridge, Colorado USA. Short Talk and poster presentation: “Imaging the Alphavirus ExitPathway”.MartinezM.G.,SnappE.L.,PerumalG.S.,MacalusoF.P.&KielianM.C.

• 2010. Invited Speaker. “SBBq-Conesul Symposyum-XXXIX AnnualMeeting of the SBBq”. “Role of L-SIGNandDC-SIGNinJuninarenavirusentryintohostcells.”Martinez,M.G.,BelouzardS.,WhittakerG.R.andCandurraN.A.FozdoIguaçu,Brazil.

Posterpresentations• 2014.AmericanSocietyofCellBiologyAnnualMeeting.Philadelphia,PA.“ImagingtheAlphavirusExitPathway”.MartinezM.G.,SnappE.L.,PerumalG.S.,MacalusoF.P.&KielianM.C.

• 2010. American Society of Cell Biology Annual Meeting. Philadelphia, PA. “S-layer proteins ofLactobacillusacidophilusinhibitoryeffectofJUNVinfection”.PradoAcostaM.,RuzalS.,CandurraN.A.&MartinezM.G.

• 2010. Annual Meeting of The American Society for Virology. Montana State University, Montana.“Utilization of C-type lectins and transferrin receptors for entry and infection of host cells by theargentine hemorrhagic fever arenavirus, Junin Virus.” M. Bialecki, M.G. Martinez, S. Belouzard, A.Regan,N.A.Candurra,G.Whittaker.

• 2009.AmericanSocietyofCellBiologyAnnualMeeting.SanDiego,CA.“RoleofL-SIGNandDC-SIGNforentryofJuninarenavirusintohostcells.”Martinez,M.G.,BelouzardS.,Candurra,N.WhittakerG.R.

• 2008.AmericanSocietyofCellBiologyAnnualMeeting.SanFrancisco,CA.“CharacterizationofJUNVarenavirusendocytosis:Requirementofcellproteins”.Martinez,M.G.,Forlenza,B.andCandurra,N.A.

• 2008.ArgentinianConferenceofVirology.BuenosAires,Argentina.“InvolvementofcellcytoskeletoninJunínvirusentryinVerocells”.MartinezM.G.,CordoS.,andCandurraN.A.

• 2007. Annual Meeting of Argentine Society for Biochemical and Molecular Biology Investigation.“Study of Junín arenavirus glycoproteins association to cholesterol enriched membrane domains”.MartinezM.G.,CordoS.andCandurra,N.A.

• 2007.1stWorkshoponAdvancedFluorescenceMicroscopy.PhysicsDepartment,SchoolofSciences,University of Buenos Aires, Argentina. “JUNV infects Vero cells by clathrin mediated endocytosis”.Martinez,M.G.,Cordo,S.andCandurra,N.A.

• 2006. A.U.G.Mmeeting.University of Campinas (UNICAMP), Brazil. “Study of endocytic pathway ofJuninvirusinVerocells”.Martinez,M.G.,Cordo,S.andCandurra,N.A.

• 2005. Argentinian Conference of Virology. Buenos Aires, Argentina. “Characterization of Junín virusentryinVerocells”.MartinezM.G.,CordoS.andCandurraN.A.

Teachingexperience2008.TeachingAssistantin“CellCultureanditsbiotechnologicalapplicationsCourse”.UniversityofBuenosAires,Argentina.2006.TeachingAssistantinGeneralChemistry.FacultyofCriminalisticsScience,UniversityoftheArgentinianFederalPolice.BuenosAires,Argentina.2005.TeachingAssistantin“MolecularbiologyeducationformedialevelWorkshop”inthe“NationalCongressofScienceandTechnologyintheNewMillennium”.SanRafael,Argentina.

TrainingCourses2015.Softwarecarpentry:Hands-onworkshopthatcoversbasiclabskillsforscientificcomputing.NewYorkAcademyofSciences.2012.Volocitytraining,PerkinElmer:VolocityQuantitationandHighcontentImageanalysis.NewYork.2012.QuantitativeImagingofCells.AlbertEinsteinCollegeofMedicine,NewYork.2010.EMBOCourse:“Microscopy:fromgenomescaletothesinglemolecule”.CSIR-Pretoria,SouthAfrica.2009.“IntroductiontoBiostatistics”.IBYME,SchoolofSciences,BuenosAiresUniversity.2009.“ConfocalMicroscopy”.NationalAcademyofMedicine.BuenosAires,2009.2008.“Introductionto3Dstructuredeterminationinvirology”.PasteurInstituteMontevideo,Uruguay.2007.“FirstSouthAmericanWorkshoponAdvancedFluorescenceMicroscopyTechniques:DynamicsThroughtheMicroscope:FluorescenceCorrelationSpectroscopy”.PhysicsDepartment.SchoolofSciences,BuenosAiresUniversity.BuenosAires.2007.“Introductiontoflowcytometry”.HematologicalInvestigationInstitute“MarianoR.Castex”,NationalAcademyofMedicine,BuenosAires.2005.“Informaticstoolsforthestructuralanalysisofnucleicacidsandproteins”BiologicalChemistryDepartment,SchoolofSciences,BuenosAiresUniversity.Honorsandawards2014. Dennis ShieldsMemorial Postdoctoral Research Poster Award. Belfer Institute for PostdoctoralResearch,AlbertEinsteinCollegeofMedicine.November2014.2010.EMBOtravelgrant:“Microscopy:fromgenomescaletothesinglemolecule”.CSIR-Pretoria,SouthAfrica.2010. Postdoctoral fellowshipprovidedby theArgentineCouncil for Scientific andTechnicalResearch(CONICET).2009.AmericanSocietyofCellBiologytravelawardtoassisttothe49thAmericanSocietyofCellBiologyAnnualMeetinginSanDiego,CA.2009.“JournalofCellScience”travelawardtoassisttothe49thAmericanSocietyofCellBiologyAnnualMeetinginSanDiego,CA.2008. “American Society for Microbiology” fellowship to make a collaborative research visit to Dr.Whittaker’sLaboratory,CornellUniversity,CollegeofVeterinaryMedicine,Ithaca,NewYork,USA2008. “Journal of Cell Science” fellowship to make a collaborative research visit to Dr. Whittaker’sLaboratory,CornellUniversity,CollegeofVeterinaryMedicine,Ithaca,NewYork,USA.2008. Doctoral fellowship provided by the Argentine Council for Scientific and Technical Research(CONICET).2006.DoctoralfellowshipprovidedbytheUniversityOfBuenosAires(UBA).2005.DoctoralfellowshipprovidedbytheNationalAgency.Languages• Spanish(native);English(fluent);French(intermediate);German(basic)Service•Ad-hocreviewer,TheJournalofVisualizedExperiments(JoVE).•ScientificadvisorforCuraEarth