OPTICAL IMAGINGOF BIOMOLECULAR TENSION
WITH SUB-100 NM RESOLUTION
Richard Park, Mentor: Yang Liu, PI: Khalid Salaita, Ph.D
Plan Intro: Focal adhesions Motivation
TFM (traction force microscopy) MTFM (molecular tension fluorescence
microscopy) SIM (structured illumination microscopy) Promising results
Focal Adhesions
Kanchanawang, P. et al., Nature, 2010, 468, 580-584
Motivation (TFM) Traction force microscopy
Hoorn, Hedde van et al., Nano Lett., 2014, 14 (8), 4257-4262
10 μm
2 μm
6.5 nN
Molecular Tension Fluorescence Microscopy
(MTFM)
Liu, Y. et al. J. Am. Chem. Soc., 2013, 135 (14), pp 5320–5323Liu, Y et al. Nano Lett. 2014, 14 (10), pp 5539-5546
Results
30 min
90 min
20 μm
20 μm
RICM Tension Response
Results
30 min 90 min
Overlay
Results
10 μm 10 μm
Tension Response
SIM results
1 95 μm
GFP-Paxillin Tension Response
SIM results
5 μm
90 min
Resolved Unresolved
GFP-Paxillin Tension Response
SIM results
2 μm
90 min
Resolved Unresolved
Molecular Tension Fluorescence Microscopy
(MTFM)
Liu, Y. et al. J. Am. Chem. Soc., 2013, 135 (14), pp 5320–5323Liu, Y et al. Nano Lett. 2014, 14 (10), pp 5539-5546
Acknowledgments Brown University Undergraduate BMES
Emory University, Dr. Khalid Salaita Group
Eunice Kennedy Shriver National Institute of Child Health and Human Development
Howard Hughes Medical Institute Science Education Program
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