OPTICAL IMAGINGOF BIOMOLECULAR TENSION

WITH SUB-100 NM RESOLUTION

Richard Park, Mentor: Yang Liu, PI: Khalid Salaita, Ph.D

Plan Intro: Focal adhesions Motivation

TFM (traction force microscopy) MTFM (molecular tension fluorescence

microscopy) SIM (structured illumination microscopy) Promising results

Focal Adhesions

Kanchanawang, P. et al., Nature, 2010, 468, 580-584

Motivation (TFM) Traction force microscopy

Hoorn, Hedde van et al., Nano Lett., 2014, 14 (8), 4257-4262

10 μm

2 μm

6.5 nN

Molecular Tension Fluorescence Microscopy

(MTFM)

Liu, Y. et al. J. Am. Chem. Soc., 2013, 135 (14), pp 5320–5323Liu, Y et al. Nano Lett. 2014, 14 (10), pp 5539-5546

Results

30 min

90 min

20 μm

20 μm

RICM Tension Response

Results

30 min 90 min

Overlay

Results

10 μm 10 μm

Tension Response

SIM results

1 95 μm

GFP-Paxillin Tension Response

SIM results

5 μm

90 min

Resolved Unresolved

GFP-Paxillin Tension Response

SIM results

2 μm

90 min

Resolved Unresolved

Molecular Tension Fluorescence Microscopy

(MTFM)

Liu, Y. et al. J. Am. Chem. Soc., 2013, 135 (14), pp 5320–5323Liu, Y et al. Nano Lett. 2014, 14 (10), pp 5539-5546

Acknowledgments Brown University Undergraduate BMES

Emory University, Dr. Khalid Salaita Group

Eunice Kennedy Shriver National Institute of Child Health and Human Development

Howard Hughes Medical Institute Science Education Program