68832499 Identificacion y Reaccion de Los Aminoacidos y Proteinas

8/16/2019 68832499 Identificacion y Reaccion de Los Aminoacidos y Proteinas

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UNIVERSIDAD NACIONAL DE SAN CRISTÓBAL DE HUAMANGA

FACULTAD DE INGENIERÍA QUÍMICA Y METALURGIA

DEPARTAMENTO ACADÉMICO DE INGENIERÍA QUÍMICA

ESCUELA DE FORMACIÓN PROFESIONAL DE INGENIERÍA QUÍMICA

LABORATORIO DE QUÍMCA ORGÁNICA

INFORME 06

IDE TIFICACIÓ Y REACCIÓ DE LOS AMI OÁCIDOS Y

PROTEI AS

SIGLA : QU-241

PROFESOR : Ing. CORDOVA MIRANDA Alcira

INEGRANES : LIMACO CARPIO! El"a#ar

GRUPO :

A$O % SEMESRE AC. : 2&&' ( II

FEC)A DE E*ECUCION : + + +

FEC)A DE ENREGA : + + +

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AYACUCHO - PERÚ

2009

I. OBJETIVOS :

I,"niicar / 0"n"r l0 a3in0ci,0 / 5r0"6na 3",ian" r"acci0n".

C0n0c"r la 5r05i",a," 6ica / 7863ica ," l0 a3in0ci,0 / 5r0"6na.

C0n0c"r alg8na 5r05i",a," ," la 5r0"6na.

II. FUNDAMENTO TEÓRICO:

I. AMINOÁCIDOS Y PROTEINAS

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LOS AMINOÁCIDOS:

S0n 8ancia crialina! cai i"35r" ," a0r ,8lc"9 i"n"n carc"r ci,0 c030 5r05i",a, ica / acii,a,

;5ica9 7863ica3"n" 0n ci,0 car;nic0 c0n! 50r l0 3"n0! 8n gr850

a3in0 50r 30lil0 ?-COO)@ / 8n gr850 a3in0 ?-N)2@ lir".

P8","n r"5r""nar" "n g"n"ral 50r N)2-C)R-COO)! i"n,0 R 8n ra,ical 0 cadena lateral carac"r6ica ,"

ca,a a3in0ci,0. E0 gr850 R 0n 38/ aria,0 7863ica3"n".

D"," "l 58n0 ," ia 7863ic0! l0 a3in0ci,0 ?AA@ 0n !"#$% $&'("!$% !$( )( '&)*$ +,"($ -( *$%"!"(

+/+. S"gn "a ,"inici;n! l0 c8ar0 8i8/"n" ,"l car0n0 ala ?C @ "n 8n a3in0ci,0 0n:

• Un gr850 car0>il0

• Un gr850 a3in0

• Un 030 ," Bi,r;g"n0

• Una ca,"na la"ral R! 78" " carac"r6ica ," ca,a AA

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http://es.wikipedia.org/wiki/Mol%C3%A9culahttp://es.wikipedia.org/wiki/Grupo_carboxilohttp://es.wikipedia.org/wiki/Grupo_aminohttp://es.wikipedia.org/wiki/Grupo_aminohttp://es.wikipedia.org/wiki/Grupo_carboxilohttp://es.wikipedia.org/wiki/Grupo_aminohttp://es.wikipedia.org/wiki/Mol%C3%A9cula


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Constitu! una !"c!#ci$n !% AA #&o%ina! c0n 8n anill0 5irr0li,6nic0! 78" 58","

c0ni,"rar" c030 8n -a3in0ci,0 78" " N-8i8i,0 50r 8 5r05ia ca,"na

la"ral ?Fig8ra ," la i#78i"r,a@.

En 0,0 l0 AA 5r0"ic0! ">c"50 "n la glicina ?Gl/@! "l car0n0 " ai3i"n ,0 "nani;3"r0

?i;3"r0 ;5ic0@! 8n0 ," la "ri" D / 0r0 ," la "ri" L. L0 AA 5r0"ic0 0n

inarial"3"n" ," la "ri" L. En alg8n0 ca0 38/ c0ncr"0 " 58","n "nc0nrar AA ," la "ri" D: "n l0

5"5i,0glican0 ," la 5ar", c"l8lar! "n ci"r0 5


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En "" ,i80 58"," "r" la ;r38la ," "ll0! "n c0l0r n"gr0 la 5ar" c03n! 3i"nra 78" "n c0l0r a#8l 58","

"r" la 5ar" arial"! 78" ,a a l0 a3in0ci,0 ,iin0 c0350ra3i"n0! la cla" ," c0l0r" " la ig8i"n":

(a) Color marrón = aminoácidos hidrófobos.

(b) Color verde = aminoácidos polares.

Color fucsia = aminoácidos ácidos.

(c) Color turquesa = aminoácidos básicos.

)a/ 78" ,"acar 78"! i ala 8n0 0l0 ," "ll0 ?A3in0ci,0 ""ncial"@ n0 "r 50il" in"i#ar ning8na ," la

5r0"6na "n la 78" "a r"78"ri,0 ,icB0 a3in0ci,0. E0 58"," ,ar l8gar a ,i"r"n" i50 ," ,"n8rici;n!

"gn c8al "a "l a3in0ci,0 li3ian".

Lista d! Aminoácidos 'Es!ncia%!s no !s!ncia%!s( )unci$n d! cada una d! !%%os:

L - A%anina: Función In"ri"n" "n "l 3"a0li30 ," la gl8c0a. La gl8c0a " 8n car0Bi,ra0 i35l" 78" "l

0rgani30 8ili#a c030 8"n" ," "n"rg6a.

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L - A&*inina: Función E i35lica,a "n la c0n"raci;n ,"l "78iliri0 ," nir;g"n0 / ," ,i;>i,0 ," car0n0.

a3iina ,"l 0rr"n" ang86n"0.

L - Cit&u%ina: Función In"ri"n" "5"c6ica3"n" "n la "li3inaci;n ,"l a30n6ac0.

L - Cistina: Función a3i


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L - ,au&ina: Función Ei38la la )0r30na ,"l Cr"ci3i"n0 ?)G)@ "n a0ciaci;n c0n 0r0 a3in0ci,0! "a

i35lica,a "n la r"g8laci;n ," la 5r"i;n ang8in"a! 0ral"c" "l 3c8l0 car,iac0 / ig0ri#a "l i"3a n"ri00.

L - ,i&osina: Función E 8n n"8r0ran3i0r ,ir"c0 / 58"," "r 38/ "ica# "n "l raa3i"n0 ," la ,"5r"i;n! "n

c03inaci;n c0n 0r0 a3in0ci,0 n"c"ari0.

L - O&nitina: Función E "5"c6ic0 5ara la B0r30na ,"l Cr"ci3i"n0 ?)G)@ "n a0ciaci;n c0n 0r0 a3in0ci,0

/a 3"nci0na,0. Al c03inar" c0n la L-Arginina / c0n carniina ?78" " in"i#a "n "l 0rgani30! la L-Orniina

i"n" 8na i350ran" 8nci;n "n "l 3"a0li30 ,"l ">c"0 ," graa c0r50ral.

L - P&o%ina: Función E in0l8cra,a a3i


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L - 0a%ina: Función Ei38la "l cr"ci3i"n0 / r"5araci;n ," l0 "i,0! "l 3an"ni3i"n0 ," ,i"r0 i"3a /

alanc" ," nir;g"n0.

M8cB0 a3in0ci,0 0r3an 5r0"6na ?a3in0ci,0 5r0"ic0@! 3i"nra 0r0 n8nca " "nc8"nran "n "lla.

0,0 l0 a3in0ci,0 78" c0350n"n 5r0"6na 5r""nan 8n car0n0 ai3


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C0350ra3i"n0 ," c8al78i"r a3in0ci,0 c8an,0 " i0ni#a. C8al78i"r a3in0ci,0 58"," c0350rar" c030

ci,0 / c030 a"! " ,"n03inan 8ancia anfóteras.

L0 a3in0ci,0 / 5r0"6na " c0350ran c030 8ancia a35;n. C8an,0 8na 30lc"58an,0 la glicina@ 50r l0 c8al 5r""nan i;3"r0.

Si "l a3in0 " a la ,"r"cBa " ,"n03ina D! i " a la i#78i"r,a L.

4u5micas2

La 78" a"can al gr850 car0>il0 ?,"car0>ilaci;n@.

La 78" a"can al gr850 a3in0 ?,"a3inaci;n@.

La 78" a"can al gr850 R.

D.NOMENCLA,URA:

L0 a3in0ci,0 78" " 0i"n"n "n la Bi,r;lii ," 5r0"6na 0n ala a3in0ci,0 "0 c0358"0 58","n

n03rar" ," ac8"r,0 c0n la r"gla ," la UVIQPA! 5"r0 3 r"c8"n"3"n" " "35l"an l0 n03r" c038n".

A8n c8an,0 "0 a3in0ci,0 ">i"n c030 i0n" ,i50lar"! RC)?N)H@COO-! 50r ra#0n" Bi;rica "

n03ran "n "l i"3a ," la VIQPA c030 " 8i"ran la 0r38la RC)?N)2@COO)

E9-,*/$:

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http://es.wikipedia.org/wiki/Ionizaci%C3%B3nhttp://es.wikipedia.org/wiki/Ionizaci%C3%B3nhttp://es.wikipedia.org/wiki/Anf%C3%B3terohttp://es.wikipedia.org/wiki/Tamp%C3%B3nhttp://es.wikipedia.org/wiki/Tamp%C3%B3nhttp://es.wikipedia.org/wiki/Punto_isoel%C3%A9ctricohttp://es.wikipedia.org/wiki/Is%C3%B3merohttp://es.wikipedia.org/wiki/Grupo_carboxilohttp://es.wikipedia.org/wiki/Grupo_carboxilohttp://es.wikipedia.org/w/index.php?title=Descarboxilaci%C3%B3n&action=edithttp://es.wikipedia.org/w/index.php?title=Descarboxilaci%C3%B3n&action=edithttp://es.wikipedia.org/wiki/Grupo_aminohttp://es.wikipedia.org/w/index.php?title=Desaminaci%C3%B3n&action=edithttp://es.wikipedia.org/wiki/Ionizaci%C3%B3nhttp://es.wikipedia.org/wiki/Anf%C3%B3terohttp://es.wikipedia.org/wiki/Tamp%C3%B3nhttp://es.wikipedia.org/wiki/Punto_isoel%C3%A9ctricohttp://es.wikipedia.org/wiki/Is%C3%B3merohttp://es.wikipedia.org/wiki/Grupo_carboxilohttp://es.wikipedia.org/w/index.php?title=Descarboxilaci%C3%B3n&action=edithttp://es.wikipedia.org/wiki/Grupo_aminohttp://es.wikipedia.org/w/index.php?title=Desaminaci%C3%B3n&action=edit


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LAS PRO,E6NAS

La 5r0"6na 0n l0 c0358"0 nir0g"na,0 3 a8n,an" ,"l 0rgani30! a la "# 78" 8n,a3"n0 3i30

," la i,a. En ""c0! ,"i,0 a la gran ari",a, ," 5r0"6na ">i"n" / c030 c0n"c8"ncia ," 8 "r8c8ra!

la 5r0"6na c835l"n 8nci0n" 83a3"n" ,i"ra! 5arici5an,0 "n 0,0 l0 5r0c"0 i0l;gic0 /

c0ni8/"n,0 "r8c8ra 8n,a3"nal" "n l0 "r" i0. D" "" 30,0! acan ac"l"ran,0 r"acci0n"

7863ica 78" ," 0r0 30,0 n0 50,r6an 5r0,8cir" "n l0 i"350 n"c"ari0 5ara la i,a ?"n#i3a@!

ran50ran,0 8ancia ?c030 la B"30gl0ina ," la angr"! 78" ran50ra 0>6g"n0 a l0 "i,0@! c835li"n,0

8nci0n" "r8c8ral" ?c030 la 78"raina ,"l 5"l0@! iri"n,0 c030 r""ra ?al3ina ," B8"0@! "c.

La 5r0"6na 0n i050l63"r0 ," l0 ala a3in0ci,09 la 5r05i",a," 6ica / 0rgnica ," 8na 5r0"6na

"n ,""r3ina,a 50r l0 a3in0ci,0 78" la c0ni8/"n. La 88ni,a," in,ii,8al" ," a3in0ci,0 "

8n"n 50r "nlac" a3i,a! lla3a,a "nlac" 5"56,ic0 la ig8ra 78" 3 a,"lan" " 38"ra9 M8"ra la "r8c8ra

g"n"ral ," 8n ala a3in0ci,0 / ," 8na 5r0"6na.

L0 ali3"n0 78" ing"ri30 n0 5r0""n 5r0"6na. P"r0 al" 5r0"6na n0 " a0r"n n0r3al3"n" "n al

c0ni8ci;n in0 78"! l8"g0 ," 8 ,",0la3i"n0 ?Bi,r;lii 0 r08ra@! ca8a,0 50r "l 5r0c"0 ," ,ig"i;n!

arai"an la 5ar", in"inal "n 0r3a ," a3in0ci,0 / ca,"na c0ra ," 5


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L0 5il0 ?-COO)@ ," 8n

a3in0ci,0 / "l gr850 a3in0 ?-N)2@ ,"l a3in0ci,0 c0nig80 in3",ia0! c0n "l c0nig8i"n","5r"n,i3i"n0 ," 8na 30l


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)a/ ,0 i50 5rinci5al" ," 5r0"6na: la i35l" 78" "n c0ni8i,a nica3"n" 50r a3in0ci,0! / la

5r0"6na c0n8ga,a 78" 0n la 78" i"n"n "n 8 c0350ici;n 0ra 30l


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ES,RUC,URA DE LAS PRO,E6NAS:

La o&*ani8aci$n ," 8na 5r0"6na i"n" ,"ini,a 50r c8ar0 ni"l" "r8c8ral" ,"n03ina,0: "r8c8ra

5ri3aria! "r8c8ra "c8n,aria! "r8c8ra "rciaria / "r8c8ra c8a"rnaria. Ca,a 8na ," "a !st&uctu&as

in0r3a ," la ,i50ici;n ," la an"ri0r "n "l "5aci0.

El 5ri3"r ni"l "r8c8ral 78" " 58"," ,"li3iar "n 8na 5r0"6na! " c0ni8i,0 an0 50r "l n3"r0 / la

ari",a, ," a3in0ci,0 78" "nran "n 8 c0350ici;n! c030 50r "l 0r,"n a3i"n,i,a! 3i"nra 78" "n 0r0 ca0 " "nr0llan "n 0r3a B"lic0i,al c030 i 0r3aran 8n

r"0r". A "" "g8n,0 ni"l " l" lla3a "r8c8ra "c8n,aria. E>i"n ,0 i50 ," "r8c8ra "c8n,aria:

1. la a?ala@-Bi30 ," "r8c8raci;n c0rr"50n," 5r"cia3"n" a 8

"r8c8ra "rciaria. C8an,0 " raa ," 8na 5r0"6na 0lig03


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r"ali#ar 8nci0n" ," ran50r" ! "n#i3ica! B0r30nal". Ea c0n0r3aci;n gl08lar " 3ani"n" "al"

gracia a la ">i"ncia ," "nlac" "nr" l0 ra,ical" R ," l0 a3in0ci,0. A5ar"c"n ari0 i50 ," "nlac":

1. "l 58"n" ,i8l8r0 "nr" l0 ra,ical" ," a3in0ci,0 78" i"n" a#8r".

2. l0 58"n" ," Bi,r;g"n0

. l0 58"n" "lr"30 0 a "35"ra8ra

"l"a,a! l" Bac" ">5"ri3"nar 8n cam1io c0n0ci,0 c030 ,"na8rali#aci;n! "l ""c0 3 iil" ,"l c8al!

c0ni" "n 8n ,"c"n0 ," 8 0l8ili,a,. P8"0 78" l0 "nlac" 7863ic0 c0al"n" ,"l "78"l"0 5"56,ic0

," la 5r0"6na n0 " r035"n ,8ran" "" raa3i"n0 r"laia3"n" 8a"! " lBa ll"ga,0 a la c0ncl8i;n ,"

78" la "r8c8ra 5ri3aria 5"r3an"c" inaca. La 3a/0r6a ," la 5r0"6na gl08lar" ">5"ri3"nan "l #&oc!so ,"

,"na8rali#aci;n c8an,0 " cali"nan 50r "nci3a ," =&K-&K C. La 0r3aci;n ," 8n c0g8l0 in0l8l" lanc0

c8an,0 " Bi"r" la clara ," B8"0 " 8n ""35l0 c03n ," ,"na8rali#aci;n


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L0 a3in0ci,0 "n a35lia3"n" ,i8n,i,0 "n la na8ral"#a. Anl0ga3"n" c030 la 30l5r"ar ," la 30li3a,a3"n".

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III. MATERIALES Y REACTIVOS:

Mat!&ia%!s:

M"cB"r0 ," 8n"n.

S"i 80 ," "na/0.

R"illa.

E58la.

Pi"a

Pin#a 5ara 80 ," "na/0

a0 ," Mar6a

R!actios:

Ean0l. C2)=O

ci,0 cl0rB6,ric0 )Cl

ci,0 n6ric0 )NO

)i,r;>i,0 ," 0,i0 al 2&. NaO)

S8la0 c5ric0 al 1. C8SO4

NinBi,rina.

Mill0n. -----

Nira0 ," 0,i0. NaNO

Al3ina. Clara ," B8"0

Ca"6na. L"cB"

Glicina. )2N-C)2COO)

Ciina. 2 - SC)2C)?N)2@COO)

A5aragina. )2NCOC)2C) ?N)2@ COO)

Liina. )2N?C)2@4C)?N)2@COO)

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IV. PROCEDIMIENTO< DATOS Y RESULTADOS E=PERIMENTAL:

ENSAYO N> 0. COAGULACIÓN:

S" ,i580 ," = 80 ," "na/0! a ca,a 8n0 ," "ll0 " "cB; 2 3L ," la 0l8ci;n ," clara ," B8"0. El5ri3"r 80 " cal"n; / " 0"r;! al "g8n,0 80 " agr"g; 4 3L ," "an0l! al "rc"r0 " aa,i; g0a," ci,0 cl0rB6,ric0 c0nc"nra,0! al c8ar0 " agr"g; ci,0 n6ric0 c0nc"nra,0 / al 78in0 8na 0l8ci;n ,"NaO) al 2&. El ">0 80 " 8; 5ara c035arar. L8"g0 " 0"r; / an0; "n l0 ca0 78" " Ba0r3a,0 la c0ag8laci;n. % " r"5ii; "l "na/0 c0n la 0l8ci;n ," ca"6na.

AL;UMINA:

REACCIONES:

O;SER0ACI3N:

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La al3ina "n "l 5ri3"r 80 ," "na/0 r"acci0na "n 5r""ncia ," cal0r! 0r3an,0 8n c0ag8l0 ," c0l0r

LANCO! "n "l "g8n,0 80 r"acci0na c0n "l "an0l 0r3an,0 8n c0ag8l0 ," c0l0r LANCO! "n "l

"rc"r 80 r"acci0na c0n aci,0 cl0rB6,ric0 0r3an,0 8n c0ag8l0 ," c0l0r LANCO! "n "l c8ar0 80

r"acci0na c0n aci,0 n6ric0 0r3an,0 8n c0ag8l0 ," c0l0r LANCO! "n "l 78in0 80 n0 B80 r"acci;n

c0n "l Bi,r;>i,0 ," 0,i0.

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CASE6NA

REACCIONES:

O;SER0ACI3N:

La ca"6na "n "l 5ri3"r 80 ," "na/0 r"acci0na "n 5r""ncia ," cal0r! 0r3an,0 8na SOLUCION

INCOLORA! "n "l "g8n,0 80 n0 r"acci0na c0n "l "an0l! "n "l "rc"r 80 r"acci0na c0n aci,0

cl0rB6,ric0 0r3an,0 8n c0ag8l0 ," c0l0r LANCO! "n "l c8ar0 80 r"acci0na c0n aci,0 n6ric0

0r3an,0 8n c0ag8l0 ," c0l0r LANCO! "n "l 78in0 80 r"acci0na c0n "l Bi,r;>i,0 ," 0,i0 0r3an,0

8na SOLUCION INCOLORA.

DISCUSI3NES:

La ,"na8rali#aci;n ," la 5r0"6na 0c8rr" c8an,0 la aracci0n" in"r30l"c8lar" ,


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"35"ra8ra 0 "n "l 5) al"ra 8 "r8c8ra "rciaria la 78" " ca8an" ," 78" la 5r0"6na "

,"na8ralic". La ,"na8rali#aci;n " ""ca "n c0n,ici0n" an 8a" 78" la "r8c8ra 5ri3aria

5"r3an"c" inaca! 5"r0 la "r8c8ra "rciaria " ,",0la ," 8na 0r3a gl08lar "5"c6ica a 8na

ca,"na "nr0lla,a al a#ar.

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ENSAYO N> 0;. REACCIONES DE BIURET.

En 8n 80 ," "na/0 " "cB; 2 3L ," 0l8ci;n ," clara ," B8"0! agr"gar 1 3L ," NaO) al 4&!l8"g0 " aa,i; g0a a g0a la 0l8ci;n ," 8la0 c5ric0 al 1! " agi; / " c0nin8; aa,i"n,0 Baa

n0ar ca3i0. L8"g0 " r"5ii; "l "na/0 c0n la 0l8ci;n ," ca"6na / c0n l0 a3in0ci,0.

REACCIONES:

As#a&a*ina:

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O;SER0ACI3N:

La al83ina! ciina / "5aracina r"acci0nan c0n "l 8la0 ," c0r" "n 3",i0 alcalin0! ,an,0 0l8ci0n"

"n ,i"r"n" 0n0 a#8l". El r"0 ," la 38"ra ?ca"ina! glicina / liina@ n0 r"acci0nan c0n "l 8la0

," c0r" 78",an,0 0l8ci0n" ," c0l0r "n 0n0 c"l"".

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DISCUSI3N:

!l reactivo de "iuret reacciona frente a las prote#nas $ aminoácidos dando a la solución una coloración

a%ul.

ENSAYO N> 0?. REACCIÓN =ANTOPROTÉICA.

S" "cB; "n 8n 80 ," "na/0 2 3L ," la 0l8ci;n ," clara ," B8"0! " aa,i; 1 3L ," ci,0 n6ric0

c0nc"nra,0! / " cal"n; "n a0 3ar6a 50r 2 ( 3in80! l8"g0 ," "nriar " aa,i; Bi,r;>i,0 ," 0,i0

al 4& Baa 78" la 0l8ci;n "a 8"r"3"n" calcina. S" 0"r; l0 ca3i0 ," c0l0raci;n / 0r0

,"all". S" r"5ii; "l "na/0 c0n 0l8ci0n" ," ca"6na / a3in0ci,0.

.

REACIONES:

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O;SER0ACI3N:

La a%1an05r0 0@. PRECIPITACIÓN DE PROTEÍNAS.

S" ,i580 ," = 80 ," "na/0! al 5ri3"r0 " "cB; T 3L ," ag8a! al "g8n,0 T 3L ," la 0l8ci;n ,"

clara ," B8"0! al "rc"r0 T 3L ," ag8a / 4 g0a ," )Cl al 1&! al c8ar0 T 3L ," 0l8ci;n ," clara ,"

B8"0 / 4 g0a ," )Cl al 1&! al 78in0 T 3L ," ag8a / 4 g0a ," 0l8ci;n ," NaO) al 1&! al ">0 T

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3L ," 0l8ci;n ," clara B8"0 / 4 g0a ," NaO) al 1&. A ca,a 80 agr"gar 2 3L ," 0l8ci;n ,"

8la0 ," c0r" al 1& / " 0"r; l0 r"8la,0.

AL;UMINA

REACCIONES:

O;SER0ACI3N:

La r"acci;n ," la al3ina r"n" al C8SO4 ?80 2@ n0 ,i0 8n 5r"ci5ia,0 ," c0l0r c"l""! r"n" al )Cl

?80 4@ 5r"ci5i; ," c0l0r a#8l clar0! / r"n" al NaO) ?80 =@ 5r"ci5i0 ," c0l0r i0l"a.

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D" "0 ,",8ci30 78" la 5r0"6na 5r"ci5ian "n 0n0 a#8l - i0l"a c0n C8SO4 "n 3",i0 ci,0 / ic0

r"5"cia3"n"! carac"ri#an,0 "" "na/0 5ara la i,"niicaci;n. ," la 5r0"6na.

CASEINA

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REACCIONES:

ENSAYO N> 0. REACCIÓN CON LA NINHIDRINA.

En 8n 80 ," "na/0 " "cB; 1 3L ," 8na 0l8ci;n ," a3in0ci,0! l8"g0 " agr"g; T g0a ," la

0l8ci;n ," NinBi,rina! " cal"n; a "8llici;n 50r 2 ( T 3in80. La c0l0raci;n a#8l 0 i0l"a n0 in,icaa

la 5r""ncia ," 8n a3in0ci,0. S" r"5ii; "l "na/0 c0n 0r0 a3in0ci,0! c0n la 0l8ci;n ," al3ina

/ c0n la 0l8ci;n ," ca"6na.

REACCIONES:

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O;SER0ACI3N:

A l0 80 1! 2! ! 4! T / = c0n"ni"n,0 al83ina! ca"ina! glicina! ciina! a5aragina / liina

r"5"cia3"n"! " l" aa,i; "l r"aci0 ," ninBi,rina / " cal"n; a "8llici;n. 0,a "a 0l8ci0n"

?"nria,a@ r"acci0nan c0n "l nira0 ," 0,i0 ,an,0 0l8ci0n" "n 0nali,a," i0l"a! c0n ">c"5ci;n ,"

la a5aragina 78" ,a 8na 8n c0l0r r00! n0 r"acci0na.

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DISCUSI3N:

La ninBi,rina / "l NaNO 0n r"aci0 78" "35l"an cal0r 5ara r"acci0nar c0n la 5r0"ina / l0

a3in0ci,0 0r3an,0 0l8ci0n" ," 0nali,a," i0l"a.

ENSAYO N> 06. REACCIÓN DE MILLON.

En 8n 80 ," "na/0 " "cB; 2 3L ," la 0l8ci;n ," clara ," B8"0! " agr"g; T g0a ,"l r"aci0 ,"

Mill0n / " cal"n; "n 8n a0 ," ag8a Biri"n,0 50r 1& 3in80! l8"g0 ," "nriar a "35"ra8ra

a3i"n"! " agr"g; T g0a ," 0l8ci;n ," nira0 ," 0,i0. La a5arici;n ," 8n c0l0r r00 la,rill0 n0

in,icaa 8n r"8la,0 50ii0. S" r"5ii; "l "na/0 c0n la 0l8ci;n ," ca"6na / c0n 0l8ci0n" ," l0

a3in0ci,0.

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REACCIONES:

O;SER0ACI3N:

La a%1


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02 CONCLUSIONES:

• A5r"n,i30 a ,i"r"nciar / r"c0n0c"r la 5r0"6na / a3in0ci,0 "n la ,iina r"acci0n"

r"ali#a,a.

• P8,i30 0"rar la ,i"r"n" r"acci0n" 78" " r"ali#ar0n 5ara "l raa3i"n0 "5"c6ic0 ,"

la 5r0"6na ?al3ina@.

• La 5r0"6na c0ni8/"n 8na ," la 30l5licaci0n" ,"l 5r0"0r "n la ,iina 5racica 78" " r"ali#a "n l0

la0ra0ri0.

•

"n"r "n c8"na 78" la "35"ra8ra " aan" i350ran" "n la c0l0raci;n / r"acci;n• R"ali#ar c0n c8i,a,0 l0 "na/0 ," la 5rcica! 58"," 0cai0nar 78"3a,8ra 8 0r0 ,a0.

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VII. CUESTIONARIO:

. D!sc&i1a ot&os dos !nsaos #a&a aminoácidos2

R!acci$n d! E&%ic:

La 5r""ncia ," anill0 ar03ic0 "n;lic0 0 nir0g"na,0 "n la ca,"na la"ral ," l0 A3in0ci,0 "

58"," i,"niicar 3",ian" la r"acci;n c0n ci,0 8lan6lic0 / niri0 ," S0,i0 50r 0r3aci;n ," al" ,"

Dia#0ni0 8"r"3"n" c0l0r"a,a 5"r3ii"n,0 a6 ,""car la 5r""ncia ," ir0ina " )ii,ina lir" 0

0r3an,0 5


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• *&$1-7(+% %",*/-%: 0r3a,a ">cl8ia3"n" 50r α-a3in0ci,0! c030 " "l ca0 ," la

8i78iina! 8na 5r0"aa inrac"l8lar 0r3a,a 50r T AA.

*&$1-7(+% !$(9)'+#+%: 78" c0ni"n"n a,"3 ," la ca,"na 50li5"56,ica 8n c0350n"n" n0

a3in0ac6,ic0 lla3a,0 gr850 5r0


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VIII. BIBLIOGRAFÍA :

• QUIMICA ORGNICA FUNDAMENAL! LOUIS F. FISER - Mar/ Fi"r. E,. AlaBa3ra ..a. Pg. 1'4 (

2&

• QUIMICA ORGNICA! *B0n Mac M0rr/. E,ici;n Ta. Pg. 4 ( ='! T4T

• QUIMICA ORGNICA. L. Finar. E,. AlaBa3ra. .a. P5 .T ( T'

• B5:WWW.ali3"naci0n-ana.c03.arin0r3aci0n"n0",a,"a3in0aci,0.B3

• B5:WWW.arrai."Xll8"ng05r0"ina.B3l

• B5:".Wii5",ia.0rgWiiA3in0CA1ci,0

• B5:WWW.#0na,i".c03n8rici0na3aci,0.B3

• B5:WWW."B8."i030l"c8laAAaa1.B3

Página
http://www.alimentacion-sana.com.ar/informaciones/novedades/aminoacidos.htmhttp://www.arrakis.es/~lluengo/proteinas.htmlhttp://es.wikipedia.org/wiki/Amino%C3%A1cidohttp://www.zonadiet.com/nutricion/amacido.htmhttp://www.ehu.es/biomoleculas/AA/aa1.htmhttp://www.alimentacion-sana.com.ar/informaciones/novedades/aminoacidos.htmhttp://www.arrakis.es/~lluengo/proteinas.htmlhttp://es.wikipedia.org/wiki/Amino%C3%A1cidohttp://www.zonadiet.com/nutricion/amacido.htmhttp://www.ehu.es/biomoleculas/AA/aa1.htm

68832499 Identificacion y Reaccion de Los Aminoacidos y Proteinas

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Transcript of 68832499 Identificacion y Reaccion de Los Aminoacidos y Proteinas