Modulo de Investigación 1

8/18/2019 Modulo de Investigación 1

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UNIVERSIDAD MAYOR DE SAN SIMÓN

FACULTAD DE CIENCIAS Y TECNOLOGÍA

CENTRO DE BIOTECNOLOGÍA

CARRERA LICENCIATURA EN QUÍMICA

" DETERMINACIÓN DE LAS CONDICIONES ÓPTIMAS DE CRECIMIENTO DE

MICROORGANISMOS HALÓFILOS TOLERANTE A HERBICIDAS”

PRESENTADO POR: Univ. S!#nn$ M#%i&'( M!()#%!*' E&+#(i!%.

TUTOR: M&+. M#%i,!( P-%! C/#+0n

MATERIA: M012(' E34!%i5!n#( 6

FECHA: 789786.

C'+/#,#5,# ; B'(ivi#


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DETERMINACIÓN DE LAS CONDICIONES OPTIMAS DE CRECIMIENTO DEMICROORGANISMOS HALÓFILOS TOLERANTE A HERBICIDA.

I. INTRODUCCIÓN

L# !v'(2+i0n /# '%*#1' '1#& (#& '%5#& 1! vi1# !n (# i!%%# +'5' (# 1iv!%&i1#1!&%2+2%#( $ 2n+i'n#( #?!&%#!)i#& 5!#,0(i+#&? 5'vi(i1#1? 1ivi&i0n +!(2(#%? ,i'(')># 1!( 1!%%'(('? #1#4#+i0n ##5,i!n!& !3%!5'& $ 52+/'& '%'& #&4!+'& !&%2+2%#(!& $ 2n+i'n!& 1! (# +-(2(#.

D2%#n! (#& @(i5#& 1-+#1#& &! /# 1!5'&%#1'


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En !&! +#&' &! /# 2i(i#1' !( 5!1i' &'(i1' HM? 4!%' /#+i!n1' #()2n'& +#5,i'& +'5':(# v#%i#+i0n 1! 4H? +'n+!n%#+i0n 1! N#C(? !54!%#2%# $ (# 2i(i#+i0n 1! /!%,i+i1#G(i'' !n 1i!%!n!& +'n+!n%#+i'n!& &2&i2$!n1' # (# G(2+' +'5' 2!n! 1!+#%,'n'.

II. OBETIVOS

• O,*!iv' G!n!%#(:

D!!%5in#% (#& +'n1i+i'n!& 04i5#& 1! +%!+i5i!n' 1! 5i+%''%)#ni&5'&/#(0i('& '(!%#n!& #( /!%,i+i1# G(i''.

• O,*!iv'& E&4!+>i+'&:

6. D!!%5in#% (# +#4#+i1#1 1! +%!+i5i!n' 1! (#& 1i!%!n!& +!4#&/#(0i(#& !&21i#1#& # 1i!%!n!& +'n+!n%#+i'n!& 1! N#C( $ 1!/!%,i+i1#.

. D!!%5in#% (# +#4#+i1#1 1! +%!+i5i!n' 1! (#& 1i!%!n!& +!4#&!&21i#1#& v#%i#n1' !( 4H $ v#%i#n1' (# +'n+!n%#+i0n 1!/!%,i+i1#.

. Ev#(2#% !( +%!+i5i!n' 1! (#& +!4#& /#(0i(#& # 1i!%!n!&!54!%#2%#& 1! in+2,#+i0n

III. MARCO TEÓRICO6. MICROORGANISMOS ETREMÓFILOS

S'n 5i+%''%)#ni&5'&


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Un' 1! ('& &i&!5#& 5& i54'%#n!& 4#%# (# i1!nii+#+i0n 1!5i+%''%)#ni&5'& !& ',&!%v#% &2 +%!+i5i!n' !n &2&#n+i#& #(i5!ni+i#&#%ii+i#(!& 4%!4#%#1#& !n !( (#,'%#'%i'. E( 5#!%i#( #(i5!ni+i' !n !(


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. MWTODOS DE SIEMBRA

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7. GLIFOSATO

E( )(i'' NK'&'n'5!i()(i+in#? CHNOP !& 2n /!%,i+i1# 1! #54(i' !&4!+%'?1!%%'((#1' 4#%# !(i5in#+i0n 1! /i!%,#& $ 1! #%,2&'&? !n !&4!+i#( ('& 4!%!nn!&. E&#,&'%,i1' 4'% (#& /'*#& $ n' 4'% (#& %#>+!&. S! 42!1! #4(i+#% # (#& /'*#&? in$!+#%&!# %'n+'& $ #(('&? ' 42(v!%i#%&! # '+'n!& +'5' /!%,i+i1# '%!&#(.

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L#& 5'(-+2(#& 1! )(i'' $ 1! &2 4%'12+' 1! 1!&+'54'&i+i0n? !( AMPA? #? ('


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- G%#1i((# 4#%# #(+'n&.- Mi+%' 4i4!# 1! 99 L- P%',!# 1! 699 5L- E&42(#.- G#.

- G2#n!&.- A()'10n.- P#4!( #(25ini'- P#%#i(5

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-

• REACTIVOS

•

- S2(#' 1! 5#)n!&i' /!4#K/i1%##1'

M)SO7XHO.- C('%2%' 1! 4'#&i' C(.- C('%2%' 1! &'1i' N#C(.- G(2+' CH6O- Hi1%03i1' 1! &'1i' N#OH.- E3%#+' 1! (!v#12%#

- P!4'n# ,#+!%i'(0)i+#- C('%2%' 1! C#(+i' 1i/i1%##1'

• C#C(KHO- B%'52%' 1! S'1i' N#B%- B#+' A)#% - E#n'( CHO- G(i''- A)2# 1!&i(#1#

•

• EQUIPOS

- In+2,#1'%#.

- C5#%# 1! (2*' (#5in#%.- A2'+(#v!.- 4H 5!%'.- B#(#n# #n#(>i+#. .- A)i#1'% 4#%# F#(+'n&- R!%i)!%#1'

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V. METODOLOGIA•

• P#%# 1!!%5in#% (#& +'n1i+i'n!& '4i5#& 1! +%!+i5i!n' 1! ('& 5i+%''%)#ni&5'&

/#(0i('& '(!%#n!& # /!%,i+i1#? &! /i' 7 4%2!,#& 2i(i#n1' +'5' 5!1i' 1!+2(iv' !( M!1i' HM? !n !( +2#( &! &2&i2$' +'5' 2!n! 1! +#%,'n' (# G(2+'4'% !( G(i'' !n 1i!%!n!& +'n+!n%#+i'n!& 699 L?69 L $ 99 L? 4'%

http://es.wikipedia.org/wiki/Carbonohttp://es.wikipedia.org/wiki/Hidr%C3%B3genohttp://es.wikipedia.org/wiki/Ox%C3%ADgenohttp://es.wikipedia.org/wiki/Carbonohttp://es.wikipedia.org/wiki/Hidr%C3%B3genohttp://es.wikipedia.org/wiki/Ox%C3%ADgenohttp://es.wikipedia.org/wiki/Carbonohttp://es.wikipedia.org/wiki/Hidr%C3%B3genohttp://es.wikipedia.org/wiki/Ox%C3%ADgenohttp://es.wikipedia.org/wiki/Carbonohttp://es.wikipedia.org/wiki/Hidr%C3%B3genohttp://es.wikipedia.org/wiki/Ox%C3%ADgeno


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'%' (#1' #5,i-n &! /i' (# v#%i#+i0n 1! (# +'n+!n%#+i0n 1! (9J?J?69J?6J $ 9J 1! N#C(?(# v#%i#+i0n 1! 4H ????69?66 $in#(5!n! (# v#%i#+i0n 1! !54!%#2%# 9C $ C

•

• PRUEBA Z6: Ui(i#n1' 5!1i' HM +'n v#%i#+i0n 1! +'n+!n%#+i0n 1! (

9J?J?69J?6J $ 9J 1! N#C( $• v#%i#+i0n 1! +'n+!n%#+i0n 1! /!%,i+i1# 699 L?69 L $ 99 L.

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• En !&# 4%2!,# &! ,2&+# 1!!%5in#% +'n


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• P#%# !&# 4%2!,# &! /#n 1!&+#%#1' (#& +'n+!n%#+i'n!& !n (#&


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6. S! 4%!4#%#%'n ('& 5!1i'& 1! 4%'12++i0n !n 2n 5#%# E%(!n5!$!% 1! 99

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+'n+!n%#+i0n 1! ( 1! JN#C(.. S! #2'+(#v' (# &'(2+i0n $ '%'& 5#!%i#(!& 1! #4(i+#+i0n !n !( 4%'+!&'

#(+'n& $ +#*#& 4!%i 1!&!+/#,(!&.. S! #)%!)' 9 5L 1! 5!1i' # +#1# #(+0n? # +#1# +2#( &! (! 2! #=#1i!n1'

(#& 1i!%!n!& +'n+!n%#+i'n!& 1!( /!%,i+i1# 699 L?69 L $ 99 L?

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(#& 1i!%!n!& +'n+!n%#+i'n!& 1!( /!%,i+i1# 699 L?69 L $ 99 L?

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• RESULTADOS DE LA PRUEBA Z:•

• En !&# 4%2!,# #( 2i(i#% (#& %!& +'n+!n%#+i'n!& 1! ( %!&#n!& 1! (# 4%2!,##n!%i'% $ v#%i#n1' !&# v! n' &'(' (# +'n+!n%#+i0n 1! /!%,i+i1#? &in' #5,i-n !(4H? &! ',&!%v0


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•

Laguna Kollpa 1 (suelo) Cepa 2 a 15%NaCl y pH=8 / a)

100μL b) 150μL c)200μL

•

• RESULTADOS DE LA PRUEBA Z:•

• En !&# 4%2!,# &! 2&' (# +'n+!n%#+i0n '4i5# !n+'n%#1# 1! ( J N#C( $ !(

4H '4i5' i)2#( # ? 4!%' %#,#*#n1' # 2n# !54!%#2%# 1! 9]C. 1#n1' +'5'%!&2(#1'


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• RESULTADOS DE LA PRUEBA Z7:• En !&# 4%2!,# &! 2&' (# +'n+!n%#+i0n '4i5# !n+'n%#1# 1! ( J N#C( $ !(

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• C!4#: L#)2n# H!1i'n1# #)2# # JN#C(?4H\ $ T\C• a) 100μL b) 150μL c)200μL

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VII. CONCLUSIONES•

• H!+/'& ('& !n$'& n!+!%i'& ? &! /# 4'1i1' v!% !( +'54'%#5i!n' 1! ('&

1i!%!n!& 5i+%''%)#ni&5'& /#(0i('& !&21i#1'& /#+i!n1' (#& %!&4!+iv#&v#%i#+i'n!& 4#%# +#1# 4%2!,#? +'54%',#n1'


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VIII. BIBLIOGRAFIA•

• A(K`#%,#n SS? A(KM2((#5 AA? A,,#& I? S#+!,%#n1 E? %'44!n&!1

RM. S#++/#%'5'ni&4'%# /#('4/i(# &4. n'v.? # n'v!( /#('4/i(i+#+in'5$+!! i&'(#!1 %'5 5#%&/ &'i( in 2a#i. In S$& B#+!%i'(#n1Ev'( Mi+%',i'( 99 : K

• Cn'v#& D? V#%)#& C? C&'n# LN? V!n' A? Ni!' . S$n/!&i& '

)($+in! ,!#in! %'5 !3')!n2& +/'(in! in /! 5'1!%#!($ /#('4/i(i+,#+!%i25 H#('5'n#& !('n)##. A44( Envi%'n Mi+%',i'( 6 7: 79K.

• /4:88aaa.&+i!('.'%).v!8&+i!('.4/4b&+%i4\&+ic#%!3d4i1\S66K997999699997

• M#+E(%'$ R.D. 67. S'5! +'55!n& 'n /! !v'(2i'n ' !3%!5'4/i(!&.

Bi'&$&!5&? : 7K• R#5>%! D.? Nin# S!%%#n' R.? '&- An'ni' S#n1'v#( T.? H'%#+i'

Mi+%''%)#ni&5'& !3%!50i('&. A+in'5i+!'& /#(0i('& !n M-3i+' R!vi&#M!3i+#n# 1! Ci!n+i#& F#%5#+-2i+#&? v'(. ? n@5. ? *2(i'K&!4i!5,%!?99? 44. K6 A&'+i#+i0n F#%5#+-2i+# M!3i+#n#? A.C. Di&%i'F!1!%#(? M-3i+'

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/4:88,i'(')i#5!1i+#.,(')&4'.+'589698985i+%''%)#ni&5'&K!3%!5'i('&./5(• E5i(i#n' A(i!%i?D!4#%#5!n' 1! Bi'!+n'(')>#:C'n%'( 1! M!(!& $

R!&i&!n+i# # H!%,i+i1#&?A)%',i'!+n'(')i#?+2%&' 96• P!&i+i1! R!&i12!& C'55i!!. 99. P!&i+i1! R!&i12!& M'ni'%in)

R!4'%. F'2%/ Q2#%!% R!4'%• %!5!% R d M!#n& NE. 99. G($4/'! #n1 )($4/'!K%!&i&#n +%'4

in!%#+i'n& ai/ %/i'&4/!%! 5i+%''%)#ni&5&. E2%'4!#n '2%n#( ' A)%'n'5$ 6: 6K66.

• P!! R!(!$?GM F%!!!. T'(!%#n+i# # /!%,i+i1#& $ +2(iv'& %#n&)-ni+'&

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• FOTOGRAFÍAS•

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• 4H 5!%'•

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• M!1i' HM #n!& 1! #2'+(#v#%


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• GUÍA DEL USO DEL AUTOCLAVE•

6. L!v#n! (# #4# 1!( !

Modulo de Investigación 1

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