Eurosurfas 2011: Jornadas Medioambiente - Anna Surribas

Aplicaciones industriales de la biocatálisis Nombre conferencia

Aplicaciones industriales de la biocatálisis

Anna Surribas Elena Genescà, Aleix Conesa, Roshan Paul, Marolda Brouta-Agnésa i Meritxell de la Varga.

Barcelona, 16th November 2011


LEITAT’s Corporate presentation

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(*) Images: applications of Leitat Technological Center to the FotCiencia’06, FotCiencia’07, FotCiencia’08 contests:

1.- Tineoella bisselliella larva adhered to a wool fiber

2.- Surface tension modified by plasma treatment in a textile surface

3- Poliamides (transversal section)

4- Scratch test of ABS (Acrilonitrilo, Butadieno, Estireno)

OBJECTIVE

R+D+2I collaboration, adding technological value and a clear adaptation and evolution to the constant market changes.


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R+D+i market oriented services


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Industrial Biotech:

R & D in biotechnological solutions with application in industrial processes; applied

microbiology and microencapsulation.

Biocatalysis

Industrial Bioprocesses

Bioproduct production

Environmental Biotechnology

Applied microbiology (process validation, biofilm models, strain isolation and characterization…)

Microbiology testing

Microencapsulation of antimicrobial products


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–

–

–


Fibres separation process

Cultivation

Water retting

Air drying

Oven drying

Grinding (siglo XVIII)

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Project substrates

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Enzymatic retting set up

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Commercial enzymes Proveedor

Texazym BFE (pectinase) Inotex

Polygalacturonase Biocon

Xylanase Biocon

Scourzyme (pectinase) Color Center

Process variables

Enzyme cocktail

Process time

Temperature

pH

Buffer / Chelant agent

Agitation type

Fiber polishing

Bath/Fiber ratio


Results of the project

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Fibers separation with enzymes (P6):

– Ecological separation process.

– Low temperature process.

– Short time process (catalyzing action of the enzyme).

Fibers obtained with the enzymatic process (P6).


Results of the project

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•

•

•

•

•

Mechanically

treated and

enzymatic

polishing of fibers.

Mechanically

treated fibers.

Hemp skin fibers polished with

enzymes.


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Substitution of conventional cotton wet processing :

(1) less harmful

(2) less aggressive towards the environment and fibre

(3) applied at mild conditions

(4) cost-effective


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Process 2: chemical processing


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- Enzymatic desizing and scouring in shorter

time.

- An alternative amylase and pululanase

(Dextrozyme) for the desizing/scouring. A

pectinase, humectant, chelating agent and buffer

KH2PO4 are used.

- Process conditions : 62ºC and 60 min at pH 4.1

- Transfer to a second bath for chemical

bleaching.

Process 2: Two step enzymatic process


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One step continuous process

- Enzymatic desizing /scouring with a

commercial thermostable amylase (Texazyme

TA10)

- Simultaneous chemical bleaching with H2O2

- Process conditions : 105ºC during 60 min.

Single step continuous enzymatic process using thermoresistent enzymes

Process 2: One step preindustrial process


SEM Images

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A B C

Scanning Electron Microscopy pictures of treated cotton fibres.

(A)Chemical desizing, scouring and bleaching

(B)Two step enzymatic process

(C) One step continuous enzymatic processing


Textile Quality

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Weight loss Whiteness

(CIE) Iodine test Drop test

Conventional Treatment 15% 63,1 OK OK

Enzymatic semicontinuous treatment

11,75% 65 OK OK

Alternative enzymatic semicontinuous treatment

13,37% 61 OK OK

Single step enzymatic process

12,57% 71 OK OK

QUALITY ASSESSMENT:

- Desizing and scouring : iodine test and the water penetration test (UNE EN 20811).

- Bleaching: CIE whiteness using DATACOLOR spectrophotometer.

- Weight loss and tensile properties of the fabrics (EN ISO 13934/1:1999)


Reutilizations and Savings

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Industrialization and Tech Transfer

and finishing of

home textiles, technical textiles for filtration and

medical textiles.

Product Laboratory trials (CIE whiteness) Autoclave (CIE whiteness)

Texazyme TA10L 72,9 58,2

Bialfa T 72,8 61,4

Aquazym SDL 69,3 57,1


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0

5

10

15

20

25

30

35

15 20 25 30 35 40 45

SFC

(%

)

Temperature (ºC)


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ENZYMATIC

INTERESTERIFICATION


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Enzyme Characteristics

E1 Commercial Immobilized non-specific enzyme

E2 Commercial immobilized specific enzyme

Variables

Enzyme type and quantity

Process time

Temperature

Fat /oil ratio

Agitation


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0

5

10

15

20

25

30

35

40

15 20 25 30 35 40 45

SFC

(%

)

Temperatura (ºC)

40:60 Fat:Oil E1

40:60 Fat:Oil E2

50:50 Fat:Oil E1

50:50 Fat:Oil E2

60:40 Fat:Oil E1

60:40 Fat:Oil E2

70:30 Fat:Oil E1

70:30 Fat:Oil E2

80:20 Fat:Oil E1

80:20 Fat:Oil E2

Desired SFC


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SMP (ºC) 30,7

-0,6

-0,5

-0,4

-0,3

-0,2

-0,1

0

0,1

-60 -40 -20 0 20 40 60 80 100

Hea

t fl

ow

(W

/g)

Temperature (ºC)

Cooling thermogram

0,15

0,2

0,25

0,3

0,35

0,4

0,45

-40 -20 0 20 40 60 80

Hea

t fl

ow

(W

/g)

Temperature (ºC)

Heating thermogram


Conclusions

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Acknowledgments

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Thank you for your attention

[email protected]

mailto:[email protected]

Eurosurfas 2011: Jornadas Medioambiente - Anna Surribas

Environment

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