4ta Reunión Annual PulseNet América Latina Buenos Aires, Argentina June , 2006

Tânia Mara Ibelli Vaz

Experience of Instituto Adolfo Lutz, Central Public Health

Laboratory, São Paulo State, Brazil , on the use of standardized

Pulsed-Field Gel Electrophoresis - PulseNet Protocol

Instituto Adolfo Lutz São Paulo, Brazil

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BRASIL SÃO PAULO STATE

Instituto Adolfo Lutz *

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Government of São Paulo State

Secretary of Health, São Paulo

Coordination of Diseases Control CCD

Center of Epidemiological

Surveillance

Instituto

Adolfo Lutz

Division of Medical Biology

Dept.

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Immunol

Section of Bacteriology

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Center of SanitarySurveillance

National Public Health Laboratories

NRLEC Regional Laboratories of Instituto Adolfo Lutz (11 Laboratories)

Private and Public Hospital Laboratories Paraná Santa Catarina RRL Central Public Health Laboratories Rio Grande do Sul Mato Grosso do Sul

Salmonella Shigella Vibrio cholerae

√ National Reference Laboratory for Escherichia coli (NRLEC)

Instituto Adolfo Lutz Central Public Health Laboratory São Paulo State, Brazil

√ Regional Reference Laboratory (RRL)

Strains to NRLEC / RRL sent by:

Characterization of Salmonella Enteritidis strains isolated from foodborneoutbreaks and from foods associated with these outbreaks

1,5,10 , molecular marker, S. Braenderup H9812; 2,3,6,7,8 and 9 , S. Enteritidis strains from humans;4, S. Enteritids from mayonnaise

1,5,10 – molecular marker S. Braenderup H9812 4,6,7,8 and 9, S.Enteritidis strains from humans; 2 and 3, S.Enteritidis from food

1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10

Fig.1A. XbaI PFGE patterns of S.Enteritidis strains

Fig 1B. XbaI PFGE patterns of S.Enteritidis strains

Characterization of Salmonella Johannesburg strains isolated from humans and foods

1 2 3 4 5 6 7 8 9 10

1,5,10 , molecular marker, Salmonella Braenderup H9812 2, 3 and 4, S. Johannesburg strains from humans; 6,7,8 and 9, S. Johannesburg strains from foods

Fig. 2. XbaI PGFE patterns of S. Johannesburg strains from humans and from foods

1 2 3 4 5 6 7 8

Salmonella Typhi strains isolated from two typhoid fever (TF) outbreaks and from sporadic cases of TF

1,8 , molecular, marker, S.Braenderup H9812; 2,3, S.Typhi from sporadic cases of TF; 4,5 STyphi from outbreak A; 6,7, S.Typhi from outbreak B

Fig.3. XbaI PFGE patterns of SalmonellaTyphi

Characterization of Salmonella I 1,4,[5],12:i:- and S.Typhimurium strains isolated from human and non- human sources (1991-2000)

1,8,15 – Molecular marker, S. Braenderup H9812 ( XbaI);2,3,4,5,6,7,9,10,11,12,13,14 , Salmonella I1,4,[5],12:i:-

Fig 4A. XbaI PFGE patterns of Salmonella I 1,4,[5],12:i:-

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Fig 4B. XbaI PFGE patterns of SalmonellaTyphimurium

1 2 3 4 5 6 7 8 9 10 11 1 13 14 15

1,8,15 – molecular marker S. Braenderup H9812 2,3,4,5,6,7,9,10,11,12,13,14 – S. Typhimurium

Characterization of Salmonella Typhimurium isolated from sporadic cases of human diseases and from non-human sources (2003-2004)

1,7,13 – molecular marker, S. Braenderup H9812 2,3,4,5,6,8,9,10,11,12 – S. Typhimurium strains

Fig.5 XbaI PFGE patterns of SalmonellaTyphimurium

1 2 3 4 5 6 7 8 9 10 11 12 13

1 2 3 4 5 6 7 8 9 10

Fig 6.XbaI PFGE patterns of E.coli O157:H7

1,5 and10- molecular marker; 2,3,4,6,7,8 and 9 – E.coli o157:H7 strains

Characterization of E.coli O157:H7

taibelli@ial.sp.gov.brvaztmi@uol.com.br

Tânia Mara Ibelli Vaz - VazTMIInstituto Adolfo Lutz Av.Dr.Arnaldo 351, 9o andarCEP 01246-902Cerqueira Cesar – São Paulo – SPBrazil